เลือกขนาด
| ขนาด/SKU | ความพร้อมจำหน่าย | ราคา |
|---|
เกี่ยวกับสินค้านี้
Quality Segment
usage
sufficient for 100 preparations, sufficient for 100 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable
input
crude DNA
detection method
SYBR™ Green
shipped in
wet ice
storage temp.
−20°C
General description
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Features and Benefits
- Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- No loading buffers or tracking dyes required for gel analysis
- SYBR™ Green I dye acts as a nonspecific reporter for real-time PCR
- SYBR™ Green I dye is inexpensive, easy to use, and highly sensitive
- Compatible with high-throughput requirements for genetic analysis of plants
- No long enzymatic digestions
- Extract stable at 4°C for at least 6 months
Other Notes
Legal Information
1 of 1
สินค้าชิ้นนี้ | |||
|---|---|---|---|
| description sufficient for 100 preparations | description sufficient for 1000 extractions, sufficient for 1000 amplifications | description sufficient for 100 extractions, sufficient for 100 amplifications | description sufficient for 100 extractions, sufficient for 100 amplifications |
| technique(s) RT-qPCR: suitable, whole genome amplification: suitable | technique(s) PCR: suitable, whole genome amplification: suitable | technique(s) PCR: suitable, whole genome amplification: suitable | technique(s) whole genome amplification: suitable, PCR: suitable |
| input crude DNA | input crude DNA | input crude DNA | input crude DNA |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 100 reactions, sufficient for 100 preparations | usage sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions | usage sufficient for 100 extractions, sufficient for 100 amplifications, sufficient for 100 reactions | usage sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| feature dNTPs included, hotstart | feature Direct PCR, hotstart, dNTPs included | feature hotstart, dNTPs included, Direct PCR | feature hotstart, Direct PCR, dNTPs included |
คำเตือน
Warning
รหัสอันตราย
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
คลาสการจัดเก็บ
10 - Combustible liquids
จุดติดไฟ (°F)
Not applicable
จุดวาบไฟ (°C)
Not applicable
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WGK 3
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