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α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Name: α(2→3,6,8,9) Neuraminidase from <I>Arthrobacter ureafaciens</I>
Brand: SIGMA

Proteomics Grade, suitable for MALDI-TOF MS

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:

9001-67-6

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-624-6

MDL number:

MFCD00131711

EC Number:

3.2.1.18 (BRENDA, IUBMB)

Specific activity:

≥25 U/vial

Pricing and availability is not currently available.

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Properties

form

lyophilized powder

Quality Segment

200

quality

Proteomics Grade

specific activity

≥25 U/vial

suitability

suitable for MALDI-TOF MS

storage temp.

2-8°C

Description

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Physical form

Lyophilized powder

Analysis Note

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Other Notes

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

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This Item	N8271	N2876	N5521
Sigma-Aldrich N3786 α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens	Sigma-Aldrich N8271 α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens	Sigma-Aldrich N2876 Neuraminidase from Clostridium perfringens (C. welchii)	Sigma-Aldrich N5521 α(2→3,6) Neuraminidase from Clostridium perfringens (C. welchii)
description Proteomics Grade, suitable for MALDI-TOF MS	description recombinant, expressed in E. coli, buffered aqueous solution	description Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder	description recombinant, expressed in E. coli, buffered aqueous solution, ≥250 units/mg protein
specific activity ≥25 U/vial	specific activity ≥135 units/mg protein	specific activity ≥1.3 units/mg solid (using 4MU-NANA), ≥0.1 units/mg solid (using mucin)	specific activity ≥250 units/mg protein
suitability suitable for MALDI-TOF MS	suitability -	suitability -	suitability -
form lyophilized powder	form buffered aqueous solution	form lyophilized powder	form buffered aqueous solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
quality Proteomics Grade	quality -	quality -	quality -

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Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

N3536
Neuraminidase 5X Reaction Buffer 1.5 mL
SDS

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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