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GE17-5442-03

Ficoll®-Paque Premium

Cytiva 17-5442-03, pack of 6 × 500 mL

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Synonym(s):
Ficoll density gradient medium, cell culture media
NACRES:
NA.25

form

liquid

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 6 × 500 mL

manufacturer/tradename

Cytiva 17-5442-03

color

Colorless to slightly yellow

pH range

5.5-7.5

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This Item
GE17-5446-02GE17-5442-02GE17-1440-02
vibrant-m

GE17-5442-03

Ficoll®-Paque Premium

vibrant-m

GE17-5446-02

Ficoll®-Paque PREMIUM 1.084

vibrant-m

GE17-5442-02

Ficoll®-Paque Premium

vibrant-m

GE17-1440-02

Ficoll® Paque Plus

color

Colorless to slightly yellow

color

Colorless to slightly yellow

color

Colorless to slightly yellow

color

Colorless to slightly yellow

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 6 × 500 mL

packaging

pack of 6 × 100 mL

packaging

pack of 6 × 100 mL

packaging

pack of 6 × 100 mL

manufacturer/tradename

Cytiva 17-5442-03

manufacturer/tradename

Cytiva 17-5446-02

manufacturer/tradename

Cytiva 17-5442-02

manufacturer/tradename

Cytiva 17-1440-02

pH range

5.5-7.5

pH range

5.5-7.5

pH range

5.5-7.5

pH range

5.5-7.5

General description

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Application

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Features and Benefits

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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25G
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Articles

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

This page shows methods to remove Percoll from a sample after centrifugation.

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocols

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Related Content

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service