Monitoring of chemical elements and oxidative damage in patients affected by Alzheimer's disease.

Monitoring of chemical elements and oxidative damage in patients affected by Alzheimer's disease.

Annali dell'Istituto superiore di sanita (2005-10-26)

Beatrice Bocca, Giovanni Forte, Francesco Petrucci, Anna Pino, Francesca Marchione, Giuseppe Bomboi, Oreste Senofonte, Franco Giubilei, Alessandro Alimonti

PMID16244393

ABSTRACT

The haematic concentration of 26 metals and the oxidative damage in 60 patients (20 males and 40 females) affected by Alzheimer's disease and 44 healthy individuals (33 males and 11 females) were compared. In patients, the following significant (p < or = 0.05) discrepancies were found: i) increment of Ca, Cd, Hg, Mg, Si and Sn, and decrement of Al, Co, Fe and Zn in serum; ii) higher concentrations of Cu, Li, Mn, Sn and Zr and lower of Fe, Hg, Mo in blood; iii) overproduction of oxidant species (SOS) and decrease of the anti-oxidant capacity (SAC) (p < or = 0.001, for both). Variables that, joined, better discriminated between patients and controls resulted to be Si, SOS, SAC, Co, Ca, Al in serum (94% of cases correctly classified) and Cu, Zr, Mo and Fe in blood (90% of cases properly categorized).

MATERIALS

Product Number

Brand

Product Description

PHR1111

Supelco

Glycine, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1006

Supelco

Sorbitol, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1104

Supelco

L-Aspartic acid, Pharmaceutical Secondary Standard; Certified Reference Material

G7513

Sigma-Aldrich

L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

93693

Supelco

L-Proline, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

697745

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Cobalt, Carbon coated magnetic, nanopowder, <50 nm particle size (TEM), ≥99%

576883

Sigma-Aldrich

Tin, nanopowder, <150 nm particle size (SEM), ≥99% trace metals basis

50848

Supelco

L-Valine, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

91515

Supelco

L-Tyrosine, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

49603

Supelco

L-Cystine, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

61506

Supelco

L-Threonine, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

255610

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Chromium, chips, thickness ~2 mm, 99.5%

266167

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357553

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Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

349151

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Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

267074

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403326

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Strontium, granular, 99% trace metals basis

200905

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366986

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Molybdenum, powder, 10 μm, ≥99.95% trace metals basis

357626

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Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

240850

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D-Sorbitol, 99% (GC)

499579

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326429

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520373

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349224

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326380

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Cobalt, foil, thickness 0.1 mm, ≥99.99%

97336

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266558

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Aluminum, wire, diam. 1.0 mm, 99.999% trace metals basis

518573

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