Stem Cell Culture

Stem Cell Culture

Stem cells have the unique ability to self-renew or differentiate into various cell types in response to appropriate signals. These properties provide stem cells with unique capabilities for tissue repair, replacement and regeneration, making them valuable research tools in regenerative medicine and stem cell therapies.

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Types and Features of Stem Cells

  1. they have unlimited self-renewal capabilities
  2. they are non-differentiated cells with unspecialized functions
  3. they can differentiate into specific cell types under the right conditions. Stem cells are broadly characterized into multipotent or pluripotent stem cells. 
Mouse embryonic stem cells grown in the presence of feeder cells and 15% ES qualified FBS (ES-009-B) containing media after 4 days of culture.

Multipotent stem cells include adult stem cells that can self-renew or to differentiate into specialized, tissue-specific cell types. Examples include hematopoietic stem cells (HSCs) that differentiate into various blood cells; mesenchymal stem cells (MSCs) that differentiate into osteoblasts, myocytes, chondrocytes and adipocytes; and neural stem cells (NSCs) that differentiate into neurons, astrocytes and oligodendrocytes.

Pluripotent stem cells can differentiate into any cell lineage. They are classified based on the tissue of origin into embryonic stem cells (ESCs), perinatal stem cells, and induced pluripotent stem cells (iPSCs). ESCs are derived from embryos and can divide indefinitely in an in vivo stem cell culture. Perinatal stem cells are derived from umbilical or placental blood or tissue, and are the most widely used pluripotent stem cells. Cord blood banking at birth is increasingly accepted as an option for treating complicated disorders later in life. The iPSCs are adult cells that are reprogrammed, or induced to behave like ESCs. The significant advantage of using iPSCs for medical applications is the reduced chance of graft rejection, since the cells are derived from the patient’s own tissues.

Stem Cell Research Applications

Stem cells occupy an active and growing area of basic science and clinical research due to their ability to self-renew and differentiate into mature cell types. Current clinical applications for stem cells include treatments for neurological and cardiovascular diseases, autoimmune disorders, cancer, wound healing, and disease modeling and drug screening. Newly discovered gene editing technologies like CRISPR may advance stem cell research and offer enormous promise in treating difficult disorders.

Stem Cell Culture Basics

Stem cells require specialized, high-quality media and expert culture techniques for propagation in the laboratory. Suboptimal stem cell culture conditions can easily lead to unwanted stem cell differentiation or to cellular senescence. Stem cell differentiation is triggered by various factors in vivo, some of which can be replicated in in vitro stem cell cultures. Some stem cell lines are immortal and can be cultured indefinitely, so it is imperative to select the right stem cell type for your research application.

Recent advances in the stem cell field have been due to the advent of CRISPR genome editing technology and 3D cell culture techniques. Advanced protocols such as those that generate organoids from iPSCs have provided scientists with more predictive in vitro “disease-in-a-dish” models.

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