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ED2SS

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
UNSPSC Code:
12352107
PubChem Substance ID:
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

grade

reagent grade

Agency

suitable for SM 4500 - NH3

Assay

98.5-101.5% (titration)

form

powder

reaction suitability

reagent type: chelator

mp

248 °C (dec.) (lit.)

solubility

H2O: soluble, clear, colorless

application(s)

agriculture
clinical research

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid (EDTA) disodium salt is an aminopolycarboxylic acid and a hexadentate ligand. It chelates metal cations to form a stable octahedral complex. It is commonly added to buffers, solutions, and even soil samples to control the concentrations of metal ions or to remove metal contaminants. Metal chelators play a crucial role in various research applications, such as biochemistry, molecular biology, and analytical chemistry. For example, EDTA is a blood anticoagulant that chelates calcium ions in the blood, this inhibits clots and is routinely used in hematological assays.

Application

Ethylenediaminetetraacetic acid disodium salt dihydrate has been used as a component of the plasma membrane resuspension buffer, inhibitor′s preservative solution (IS) and luciferase activity assay mix.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

Reagent grade for general lab use

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Isolation of plasma membrane-associated membranes from rat liver
Suski JM, et al.
Nature Protocols, 9(2), 312-312 (2014)
Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes
Antonelli G, et al.
Clinical Biochemistry, 40(8), 545-550 (2007)
Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro.
Roelofs MJE, et al.
Toxicology Reports, 1, 271-283 (2014)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)
Environmental fate and microbial degradation of aminopolycarboxylic acids
Bucheli-Witschel M and Egli T
FEMS Microbiology Reviews, 25(1), 69-106 (2001)

Protocols

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

Enzymatic Assay of Superoxide Dismutase

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