Although not validated, many users choose to aliquot the Accumax into single use vials after the initial thaw to store frozen at -20 °C until the expiration date.
A7089
Accumax™ solution
sterile-filtered, suitable for cell culture
Synonym(s):
Cell detachment solution
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About This Item
Concentration:
1 ×
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Level
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
Application
Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
TM=["Accumax"] solution has been used:
- for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
- for dissociation of retinal pigmented epithelial cells prior to passaging
- for dissociation of islets from pancreas
Biochem/physiol Actions
Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.
Features and Benefits
Ready-to-use sterile liquid for in vitro cell applications
Preparation Note
Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.
This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
Disclaimer
Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form solution | form liquid | form liquid | form solution |
| concentration 1 × | concentration 400-600 units/mL | concentration - | concentration 1 × |
| pH 6.8-7.8 | pH 6.8-7.8 | pH - | pH 7.0-9.0 |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile; sterile-filtered |
| storage temp. −20°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. −20°C |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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After thawing Accumax, can I make aliquots and freeze them?
1 answer-
Helpful?
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Is your accumax gmp grade and made in a gmp compliant facility and is animal free?
1 answer-
This Accumax™ solution does not contain mammalian- or bacterial-derived products. It is not a GMP grade. This product is assigned a quality level of 200. Products with quality level of 200 are not suitable for GMP. Products with quality levels 400 or higher are suitable for GMP.
Currently, there is no GMP grade product available.Please see the quality brochure below for more information:
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What is the size of the bottle in size specifications (inches, centimeters)?
1 answer-
See below for the 100 mL and 500 mL bottle dimensions:
100 mL
Height: 104.1 mm
Height w/cap: 108.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 37.3 mm500 mL
Height: 173.2 mm
Height w/cap: 177.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 38 mmHelpful?
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What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
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Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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