Skip to Content
Merck
All Photos(2)

Key Documents

P7962

Sigma-Aldrich

Monoclonal Anti-PSTAIR antibody produced in mouse

clone PSTAIR, ascites fluid

Synonym(s):

Anti-CDC2

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PSTAIR, monoclonal

mol wt

antigen 33-34 kDa

contains

15 mM sodium azide

species reactivity

mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:4,000 using a COS-7 cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDC2(983)
mouse ... Cdc2a(12534)

General description

In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21.

Immunogen

synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.

Application

Monoclonal Anti-PSTAIR antibody produced in mouse has been used in:
  • western blot analysis
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoprecipitation

Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.

Biochem/physiol Actions

Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fission yeast CSL proteins function as transcription factors
Oravcova M, et al.
Testing, 8(3), e59435-e59435 (2013)
Martin Převorovský et al.
PloS one, 10(9), e0137820-e0137820 (2015-09-15)
Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped. Using a combination of transcriptome profiling
Yuko Takayama et al.
Scientific reports, 6, 38111-38111 (2016-12-03)
Histone gene expression is regulated in a cell cycle-dependent manner, with a peak at S phase, which is crucial for cell division and genome integrity. However, the detailed mechanisms by which expression of histone genes are tightly regulated remain largely
Jing Xue et al.
Cell research, 27(12), 1503-1520 (2017-11-22)
Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single- and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by
Marie Knockaert et al.
Oncogene, 21(42), 6413-6424 (2002-09-13)
Chemical inhibitors of cyclin-dependent kinases (CDKs) have a great therapeutic potential against various proliferative and neurodegenerative disorders. Intensive screening of a combinatorial chemistry library of 2,6,9-trisubstituted purines has led to the identification of purvalanol, one of the most potent and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service