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Enzyme activity assays

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Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Assay Procedure for Invertase
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Enzymatic Assay of Neuraminidase
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Enzymatic Assay of Aprotinin
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Pull-down assays
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Assay Procedure for Pyruvate Oxidase Bacterial
Protocol for Assay Procedure for Pyruvate Oxidase Bacterial
Recycling Micro-Assay of β-NAD and β-NADH
Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Superoxide Dismutase
LPL Activity Assay Protocol
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
Enzymatic Assay of Fumarase
Enzymatic Assay of Fumarase
Enzymatic Assay of Invertase
Enzymatic Assay of Invertase
Assay Procedure for Lipase
Assay Procedure for Lipase
Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
MTT Assay Protocol for Cell Viability and Proliferation
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)
Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.
Enzymatic Assay of Chloramphenicol Acetyltransferase
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
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