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Enzyme activity assays

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Drug Conjugate Analysis using β-Glucuronidases
Drug conjugate analysis and the enzymatic hydrolysis of glucuronides
Enzyme Commission Numbers
Complete List of Enzyme Commission Numbers for metabolics research.
Aprotinin
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Mucins, Glycosylated Proteins, Properties & Diagram
Mucins are a diverse family of densely glycosylated proteins. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline enabling post-translational O-glycosylation.
Neuropeptidases
We offers many products related to neuropeptidases for your research needs.
Phosphodiesterases
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.
Horseradish Peroxidase (HRP) Enzymes
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
Glycobiology - Carbohydrate Enzymes and Proteins
Explore our complex carbohydrate proteins, enzyme products and kits for investigating oligosaccharides, polysaccharides, and numerous additional aspects of your carbohydrate workflow.
Nuclear Receptors: PPARs
Peroxisome proliferator activated receptors (PPARs) are ligand-activated transcription factors related to hormone receptors, influencing gene expression.
Phospholipase C
Phospholipase C hydrolysis of PIP2 regulates cell functions, crucial in extracellular signaling pathway regulation.
Phospholipase D
PLD enzymes catalyze PC hydrolysis, influencing substrate recognition and lipid metabolism.
HMG-CoA Reductase (HMGR) Assay Kit
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
Tubulin
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.
Enzymatic Assay of Cathepsin B
Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.
Protein Kinase C (PKC)
Protein kinase C (PKC) is an AGC kinase that phosphorylates serine and threonine residues in many target proteins.
PKA & PKG
Our offers many products related to PKA & PKG for your research needs.
InsR
We offers many products related to InsR for your research needs.
Neurotensin Receptors
Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.
Enzymes for Alternative Energy
Explore the hurdles in unlocking glucose energy from lignocellulosic biomass, surpassing challenges compared to starch.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
Cholesterol Esterification
Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.
LOPAC®1280 Small Molecules Screening
Explore compound library screening options with our Pharmacologically Active Compounds portfolio.
Fibrinogen/Fibrin
Fibrinogen, an acute phase glycoprotein, plays a vital role in the coagulation cascade of proteins.
Coenzyme A
Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.
Heterotrimeric G Proteins
Heterotrimeric G proteins modulate intracellular signals from 7TM receptors, impacting various cellular functions and drug targets.
Decorin
Decorin's role in connective tissues underscores its importance in skeletal system extracellular matrix composition.
Dipeptidyl Peptidase IV
Native DPPIV, a type II transmembrane glycoprotein, functions as a serine protease in the S9 prolyl-oligopeptidase family.