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Nucleic acid labeling and detection

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facet applications:Nucleic acid labeling and detection
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NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
DIG Easy Hyb™ Granules Protocol & Troubleshooting
DIG Easy Hyb™ Granules Protocol & Troubleshooting
NBT/BCIP Stock Solution Protocol & Troubleshooting
NBT/BCIP Stock Solution Protocol & Troubleshooting
DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
Oligonucleotide Melting Temperature
Learn about methods for calculating oligonucleotide melting temperature (Tm).
Dextran Sulfate
Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
DIG RNA Labeling Mix Protocol Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
NBT Protocol
NBT Protocol
NBT Protocol & Troubleshooting
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.
Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
DIG Northern Starter Kit Protocol & Troubleshooting
DNA probes, like PCR or random-primed DNA probes, are effective for northern blots, particularly for detecting abundant messages.