Transfection and gene editing

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Simplicon™ RNA Transfection and Electroporation Protocols

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids

This procedure describes the preparation of Cationic Lipid/Oligomer Complex.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Liposome Preparation - Avanti® Polar Lipids

Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti^® Polar Lipids

This procedure describes the preparation of cationic liposomes and transfection of cells.