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Belysa® Immunoassay Curve Fitting Software

Image of scientist using Belysa® Immunoassay Curve Fitting Software, available for analyzing data from Luminex® xMAP® platforms (xMAP® INTELLIFLEX, Luminex® 200™, FLEXMAP 3D®, MAGPIX® instruments) and the SMCxPRO® ultrasensitive analyzer.

Belysa® Immunoassay Curve Fitting Software

Whether it’s Luminex® multiplex assays, SMC® kits, or ELISAs, Belysa® Immunoassay Curve Fitting Software is designed to give you confidence in your data. Our Belysa® analysis software provides curve fitting capabilities with .csv output files from the Luminex® xMAP® platforms like the xMAP® INTELLIFLEX, Luminex® 200™, FLEXMAP 3D®, and MAGPIX® instruments as well as the SMCxPRO® ultrasensitive analyzer.

Designed using current methods and principles, easy-to-use Belysa® software provides an environment to fit data appropriately based on individual standard curves within a multiplexed immunoassay, indicators of data hygiene to enhance experimental integrity and reproducibility, and a user-friendly tool for data evaluation before using it to model a biological outcome. 


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Easily Visualize Standard Curves

The Belysa® analysis software displays single and multiplex data that allows the user to immediately visualize their standard curve and the distribution of standard points, controls, and samples. Further information, derived from the curve, is provided along with the option to refit the curves through the Belysa® software algorithms. Changes to interpolated data are instantly re-calculated following optimization (e.g. the slope equation, lower limit of quantification (LLOQ), or reported sample values).

Additionally, the detailed view within the software allows the parameters of the assay well to be observed. Two sets of identifiers allow users to draw attention to a potential deviation, a warning for software-defined rules, such as Non-Detectable (ND) samples, or user-defined parameters (e.g. % Recovery).

Visualize immunoassay standard curves with Belysa® curve fitting analysis software

Figure 1.Easily visualize standard curves with Belysa® software.

Quickly Test Parallelism Between Different Runs And Compare Standard Curves

For researchers looking to compare standard curves, Belysa® software provides the option to test the mathematical parallelism between different runs of the same assay. The assumption being that if the curves are similar, then samples would report similarly if included on the other assay. A second metric of Relative Potency is also provided for those who wish to examine the consistency of a test material compared to a standard version.

Furthermore, Belysa® software offers the option to visualize parallel curves with multiplex data and allows the user to quickly see the relationship between the curves. The calculated value for this feature is conveniently presented in the lower window and reportable, both graphically and numerically, in an output file.

Taken together, these features provided by the Belysa® software aid researchers in their ability to examine each individual assay and confirm that it was successfully executed. If the assay is performed as part of a multi-plate or longitudinal study, our Belysa® software allows you to confirm the consistency of your method and that the data generated is free from experimental deviation which may affect your end results.

Test parallelism between runs and compare your immunoassay standard curves with Belysa® data analysis tools.

Figure 2. Effortlessly test parallelism and standard curves with the Belysa® data analysis tools.


  • Curve fit optimization (4pl, 5pl, Linear, Competitive, Cubic Spline)
  • Single and multi-analyte views
  • Robust auto-flagging and data hygiene features
  • Relative potency analysis for multiple compare curves
  • Modern look and feel
  • Flexible license mechanism. Activation dongle opens any copy.

System Requirements

  • PC with: 
    o   Microsoft Windows 10 or higher
    o   macOS 
  • A free USB port on the USB
  • A Belysa® license activation dongle

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