[跳轉至內容](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/TW/zh) 產品 購物車0 TWZH 產品 產品應用服務資源支援 [登入 / 註冊](https://www.sigmaaldrich.com/oidc-sign-in) [訂單查詢](https://www.sigmaaldrich.com/TW/zh/order-lookup) [快速下訂](https://www.sigmaaldrich.com/TW/zh/quick-order) 購物車0 [首頁](https://www.sigmaaldrich.com/TW/zh)[應用](https://www.sigmaaldrich.com/TW/zh/applications)[分析化學](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry)[樣品製備技術](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/sample-preparation)固相萃取(SPE) # 固相萃取(SPE) ![Visiprep™ SPE 真空集流器配備閥門系統,可精確控制流速,並使萃取結果更具重複性。](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/analytical-chemistry/spe-vacuum-manifold.jpg "SPE Vacuum Manifold") 固相萃取(SPE)是一種專為在色譜分析(例如 HPLC、GC、TLC)前進行快速、選擇性的樣品前處理與純化而設計的技術。在 SPE 過程中,透過萃取、分配及/或吸附至固相固定相,將液體樣品中的一種或多種分析物分離出來。 * * * ## 相關產品 Slide 1 of 22 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QuEChERS](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/analytical-sample-preparation/spe-12-port-vacuum-manifold.jpg "鎳鈦合金芯 SPME 纖維")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [固相萃取(SPE)與 QuEChERS](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) 探索適用於固相萃取(SPE)及 QuEChERS 樣品前處理的各式材料。提供柱、試管、板等各類產品。 [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [![各式高效液相層析(HPLC)及超高效液相層析(UHPLC)產品](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/analytical-chromatography/high-performance-liquid-chromatography-products.jpg "HPLC 與 UHPLC 產品")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/hplc-columns) [高效液相色譜柱](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/hplc-columns) 透過我們豐富多樣的 HPLC 色譜柱系列,實現精確的分離。提升保留、分離度與選擇性。立即訂購。   [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/hplc-columns) [![氣相色譜柱、配件及吸附劑](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/analytical-chromatography/crimp-top-vials.jpg "氣相色譜柱、配件及吸附劑")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) [氣相色譜柱、配件及吸附劑](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) 使用我們的色譜柱、耗材及配件,實現卓越的氣相色譜 (GC) 性能。探索 Supelco® 的產品系列。 [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) * * * 概覽 相關文章與操作規範 支援 固相萃取(SPE)是一種專為在色譜分析(例如 HPLC、GC、TLC)前進行快速、選擇性樣品前處理與純化而設計的技術。在 SPE 過程中,透過萃取、分配及/或吸附至固相固定相,將液態樣品中的一種或多種分析物分離出來。   透過 SPE 進行樣品前處理,可將樣品的原始基質轉變為更簡單的基質環境。這使樣品更適合後續的分析色譜分析,通常能簡化並提升最終的定性與定量分析效果。較簡單的樣品基質亦能降低對分析系統的要求,進而可能延長系統的使用壽命。 一套最佳的 SPE 程序可讓您: - 轉換樣品基質,使其更符合目標色譜分析方法的要求。 - 濃縮分析物(痕量富集),以提高靈敏度。 - 去除在色譜分析過程中導致高背景、誤導性峰和/或靈敏度不佳的干擾物質。 - 保護分析色譜柱免受污染物影響。 - 將萃取過程自動化。 ## SPE 是如何運作的? 在 SPE 中,固定相(吸附劑或樹脂)透過強而可逆的相互作用,與分析物或雜質結合,從而可靠且迅速地從複雜樣品中萃取目標分析物。 SPE 兼具選擇性與多功能性,因其可針對不同分析物與基質選用多種不同的吸附劑及洗脫條件。常見的 SPE 吸附劑包括: - 以二氧化矽為基礎 - 反相(C18、C8、氰基、苯基) - 正相(二氧化矽、二醇、NH₂) - 離子交換(SAX、WCX、SCX) - 碳基 - 聚合物基(各種組成、不同官能團) - 其他,例如 Florisil®(矽酸鎂)或氧化鋁 - 混合床:幾乎可將上述任何材料以連續層狀形式組合 ## 固相萃取(SPE)策略 在「吸附-洗脫 SPE」中,目標分析物被吸附劑捕獲,而基質干擾物則通過色譜柱。在「干擾物去除 SPE」中,基質干擾物被吸附劑捕獲,而目標分析物則通過色譜柱。HybridSPE 與 [](https://www.sigmaaldrich.com)[QuEChERS](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/sample-preparation/quechers) SPE 方法均採用干擾物去除原理。 最佳的 SPE 方法取決於分析物的結構、溶解度、極性及親脂性(分配係數)。現有[](https://www.sigmaaldrich.com)選型指南可協助針對特定應用選擇最適合的固定相與溶劑。 ## SPE 的常見應用 固相萃取(SPE)廣泛應用於製藥、臨床及高通量診斷檢測、法醫、環境,以及食品/農藥化學等產業,用於以下相關分析: - 生物體液中的藥用化合物及代謝物 - 生物體液中的濫用藥物 - 飲用水與廢水中的環境污染物 - 食品/農業基質中的農藥、抗生素或黴菌毒素 - 蛋白質與胜肽的脫鹽 - 脂質的分餾 - 水溶性及脂溶性維生素 [![文件搜尋](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/ecommerce/document-search.png "文件搜尋")](https://www.sigmaaldrich.com/documents-search) [想了解更多具體資訊嗎?](https://www.sigmaaldrich.com/documents-search) 請造訪我們的文件搜尋頁面,查閱產品資料表、認證書及技術文件。 [搜尋文件](https://www.sigmaaldrich.com/documents-search) ## 相關文章 - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. - [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. - [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. - [Analysis of Pesticide Residues in Pistachios Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-pistachios) Pistachios 45% fat complicates pesticide analysis. But Z-Sep+ sorbent cleanup after QuEChERS extraction can reduce background for reliable results. - [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed. - [查看全部 (41)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ## 相關協定 - [Empowering Biochemists to Analyze Glycoproteins with Exceptional Reproducibility](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns) Fast, high-resolution separations of procainamide labeled glycans - dextran ladder, human IgG and cetuximab - [Biological Testing for Amphetamine and Methamphetamine Abuse](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/biological-testing-for-amphetamine-and-methamphetamine-abuse) In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine. - [Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/chiral-hplc-amphetamine-methamphetamine) Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine - [UHPLC/MS for Drug Detection in Urine](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/uhplc-analysis-drugs-glucuronidase) Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration. - [Acrylamide in Food](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/acrylamide-in-food) Acrylamide analysis has been a very hot topic since the chemical was identified in food in 2002 by researchers at the Swedish National Food Administration. - [查看全部 (32)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ### 查看更多文章與操作規範 輸入關鍵字 搜尋 ## 我們能為您提供什麼協助 如有任何疑問,請提交[客戶支援請求](https://www.sigmaaldrich.com/TW/zh/support/customer-support) 或聯絡我們的客戶服務團隊: 電郵至 custserv@sial.com 或致電 +1 (800) 244-1173 ## 額外支援 - [色譜圖搜尋](https://www.sigmaaldrich.com/chromatogram-search) 請使用「色譜圖搜尋」功能來鑑定樣本中的未知化合物。 - [計算機與應用程式](https://www.sigmaaldrich.com/TW/zh/support/calculators-and-apps) Web Toolbox - 用於分析化學、生命科學、化學合成和材料科學的科學研究工具和資源。 - [客戶支援請求](https://www.sigmaaldrich.com/TW/zh/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [常見問題](https://maestro.my.site.com/knowledgeportal/s/) 請瀏覽我們的「常見問題」,以獲取有關本公司產品與服務的常見問題解答。 * * * __精選文章__ [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. 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