[跳轉至內容](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/TW/zh) 產品 購物車0 TWZH 產品 產品應用服務資源支援 [登入 / 註冊](https://www.sigmaaldrich.com/oidc-sign-in) [訂單查詢](https://www.sigmaaldrich.com/TW/zh/order-lookup) [快速下訂](https://www.sigmaaldrich.com/TW/zh/quick-order) 購物車0 [首頁](https://www.sigmaaldrich.com/TW/zh)[應用](https://www.sigmaaldrich.com/TW/zh/applications)[分析化學](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry)[樣品製備技術](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/sample-preparation)固相微萃取(SPME) # 固相微萃取(SPME) 固相微萃取(SPME)是一種創新且靈敏的無溶劑樣品前處理技術。SPME 基於吸附/吸收與脫附的原理,利用塗層纖維從樣品中濃縮揮發性及半揮發性化合物。 * * * ## 相關產品 Slide 1 of 16 1 of 4 [![SPME 光纤支架 for use with Manual Sampling, pk of 1](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/161/835/03e6adaf-e8d9-4c66-996a-bff08005023f/640/03e6adaf-e8d9-4c66-996a-bff08005023f.jpg) \ Supelco \ 57330-U \ SPME 光纤支架](https://www.sigmaaldrich.com/TW/zh/product/supelco/57330u) 快速查看 [![SPME 光纤支架 for use with CTC CombiPAL, Gerstel MPS2 Autosamplers, pk of 1](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/161/835/03e6adaf-e8d9-4c66-996a-bff08005023f/640/03e6adaf-e8d9-4c66-996a-bff08005023f.jpg) \ Supelco \ 57347-U \ SPME 光纤支架](https://www.sigmaaldrich.com/TW/zh/product/supelco/57347u) 快速查看 [![SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB) 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/161/835/03e6adaf-e8d9-4c66-996a-bff08005023f/640/03e6adaf-e8d9-4c66-996a-bff08005023f.jpg) \ Supelco \ 57310-U \ SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB)](https://www.sigmaaldrich.com/TW/zh/product/supelco/57310u) 快速查看 [![SPME Overcoated Fiber Assembly 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, notched pink 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1](https://www.sigmaaldrich.com/TW/zh/product/supelco/57285u) 快速查看 [![固相微萃取萃取头组合套件 2 Assortment #2, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/161/835/03e6adaf-e8d9-4c66-996a-bff08005023f/640/03e6adaf-e8d9-4c66-996a-bff08005023f.jpg) \ Supelco \ 57321-U \ 固相微萃取萃取头组合套件 2](https://www.sigmaaldrich.com/TW/zh/product/supelco/57321u) 快速查看 [![SPME 纤维分类套件 4 Assortment #4, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/161/835/03e6adaf-e8d9-4c66-996a-bff08005023f/640/03e6adaf-e8d9-4c66-996a-bff08005023f.jpg) \ Supelco \ 57325-U \ SPME 纤维分类套件 4](https://www.sigmaaldrich.com/TW/zh/product/supelco/57325u) 快速查看 [![固相微萃取便携式现场采样器 coating PDMS/DVB](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/347/971/a3f6da9b-5402-403a-849b-05dc3bf887d9/640/a3f6da9b-5402-403a-849b-05dc3bf887d9.jpg) \ Supelco \ 57359-U \ 固相微萃取便携式现场采样器](https://www.sigmaaldrich.com/TW/zh/product/supelco/57359u) 快速查看 [![Smart SPME Fiber Assembly 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 1, black hub](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/155/268/c1a818bb-c9a6-4778-97f4-c18ff1262e36/640/c1a818bb-c9a6-4778-97f4-c18ff1262e36.jpg) \ Supelco \ 548550-U \ Smart SPME Fiber Assembly](https://www.sigmaaldrich.com/TW/zh/product/supelco/548550u) 快速查看 [![智能SPME萃取头组件 50µm/30µm PDMS/DVB/CAR, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 1, light gray hub](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/183/146/9adcd9c4-d8d7-4776-8d28-a72aa9c729f7/640/9adcd9c4-d8d7-4776-8d28-a72aa9c729f7.jpg) \ Supelco \ 548675-U \ 智能SPME萃取头组件](https://www.sigmaaldrich.com/TW/zh/product/supelco/548675u) 快速查看 [![SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS) 75 μm CAR/PDMS, NIT (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub 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(metallic)](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/387/700/4c6d8fb7-8f2a-431f-8f12-03d4e88c7ee6/640/4c6d8fb7-8f2a-431f-8f12-03d4e88c7ee6.jpg) \ Supelco \ 57921-U \ SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB)](https://www.sigmaaldrich.com/TW/zh/product/supelco/57921u) 快速查看 [![SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS) 75 μm CAR/PDMS, NIT (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub (metallic)](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/775/935/e0e6a05f-72fc-47ea-9c0a-821081bb2e34/640/e0e6a05f-72fc-47ea-9c0a-821081bb2e34.jpg) \ Supelco \ 57907-U \ SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS)](https://www.sigmaaldrich.com/TW/zh/product/supelco/57907u) 快速查看 [![Smart SPME Fiber Assembly 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 1, red hub](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/images/127/093/dfc7017b-27a7-4a56-a377-b163802b4e26/640/dfc7017b-27a7-4a56-a377-b163802b4e26.jpg) \ Supelco \ 548575-U \ Smart SPME Fiber Assembly](https://www.sigmaaldrich.com/TW/zh/product/supelco/548575u) 快速查看 * * * ## 精選類別 [![分析色譜法](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/analytical-chromatography/glass-vials-with-graduation.jpg "分析色譜法")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography) [分析色譜法](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography) 探索我們的優質產品,助您在高效液相層析(HPLC)、氣相層析(GC)、卡爾·費歇爾滴定等領域獲得精準結果。從微量分析到安全設備,我們都能滿足您的需求。 [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography) [![固相微萃取(SPME)](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers.jpg "鎳鈦合金芯 SPME 纖維")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) [固相微萃取(SPME)](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) 探索先進的 SPME 解決方案:智慧纖維、鎳鈦合金芯纖維、BioSPME,實現高效的無溶劑樣品前處理。 [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-sample-preparation/spme-fibers-and-accessories) [![氣相色譜柱、配件及吸附劑](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/categories/analytical-chromatography/crimp-top-vials.jpg "氣相色譜柱、配件及吸附劑")](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) [氣相色譜柱、配件及吸附劑](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) 使用我們的色譜柱、耗材及配件,實現卓越的氣相色譜 (GC) 性能。探索 Supelco® 的產品系列。 [瀏覽商品](https://www.sigmaaldrich.com/TW/zh/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) * * * 概覽 相關文章與操作規範 支援 SPME 廣泛應用於涉及環境、生物及藥品樣本、食品與飲料、香精與香料、法醫與毒理學以及產品檢測等各類領域。典型應用包括: - 水與空氣樣本的環境分析 - 聚合物及固體樣品中微量雜質的頭空間分析 - 十億分之一級別的氣味分析 - 食品的風味分析 - 縱火及爆炸物樣本的法醫分析 - 血液酒精濃度、尿液及血清中藥物成分之毒理學分析 ## SPME 是[](https://www.sigmaaldrich.com)如何運作的? SPME 採用塗有萃取相的纖維:該萃取相可以是液體(聚合物)、固體(吸附劑),或是兩者的組合。塗覆好的纖維置於保護針內,並固定在形似注射器的固定座上。 當纖維接觸樣本時,樣本中的分析物會從樣本基質中分配至固定相,直至達到平衡狀態。纖維的塗層會透過吸收(液態塗層)或吸附(固態塗層)的方式,從樣本中萃取化合物。 經過預定的萃取時間後,將纖維取出並直接插入色譜儀器(通常為[氣](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/gas-chromatography)[](https://www.sigmaaldrich.com)[相色譜法(GC)](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/gas-chromatography)或[高效液相色譜法(HPLC)](https://www.sigmaaldrich.com/TW/zh/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc))中,進行脫附與分析。在 GC 中,分析物的脫附是透過加熱進行的;而 HPLC 則使用溶劑將分析物脫附至液相中。 ## SPME 的[](https://www.sigmaaldrich.com)優勢 SPME 將分析物的採樣、分離與富集整合為單一、簡單的步驟。透過控制纖維塗層的極性與厚度、維持恆定的採樣時間,以及控制其他幾項萃取參數,SPME 使分析人員能夠確保從樣品中獲得高度一致且可量化的結果,即使分析物濃度較低亦然。 SPME 的其他優點包括: - 無需使用溶劑 - 易於自動化 - 對樣品無破壞性 - 適用於幾乎任何樣品或基質 - 所使用的纖維可重複使用且價格低廉 - 纖維體積小,適合現場作業 - 相容於氣相色譜(GC)或高效液相色譜(HPLC)儀器 ## 用於氣相色譜分析的 [](https://www.sigmaaldrich.com)SPME 纖維 傳統的 SPME 技術用於萃取並濃縮分析物,以供氣相色譜分析之用。萃取方式可分為直接浸入法(DI-SPME)——即將纖維直接浸入液體樣品中;或頭空間 SPME(HS-SPME)——即將纖維置於樣品上方的蒸氣相中。 ## 適用於液相色譜-質譜(LC-MS)分析的 [](https://www.sigmaaldrich.com)Supel™ BioSPME Supel™ BioSPME 是一種生物分析微量取樣與樣品前處理技術,用於從生物樣品中快速且選擇性地萃取廣泛的分析物,同時排斥不需要的大分子(例如:脂質、蛋白質)。後續分析通常透過 LC-MS 進行。Supel™ BioSPME 透過直接萃取運作,無需樣品預處理,並提供非耗盡式、基於平衡的萃取。 [![文件搜尋](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/ecommerce/document-search.png "文件搜尋")](https://www.sigmaaldrich.com/documents-search) [想了解更多具體資訊嗎?](https://www.sigmaaldrich.com/documents-search) 請造訪我們的文件搜尋頁面,查閱產品資料表、認證書及技術文件。 [搜尋文件](https://www.sigmaaldrich.com/documents-search) ## 相關文章 - [Selection Guide for Supelco SPME Fibers](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-spme-fibers) Recommended fibers based on analyte type and molecular weight; tips for SPME fiber selection for a variety of applications. - [Supel™ BioSPME 96-Pin Devices](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/supel-biospme-96-pin-devices) Supel™ BioSPME 96-Pin devices enhance speed and simplicity for plasma protein binding studies and LCMS analyses. - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) Answers and tips relating to the application of the solid phase micro-extraction technique. - [Rugged SPME Sampling](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) SPME fibers with CAR/PDMS and PDMS/DVB coatings on nitinol core assembly enhance fiber performance for solid phase microextraction. - [Selecting the Appropriate SPME Fiber Coating –Effect of Analyte Molecular Weight and Polarity](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-the-appropriate) Selecting the Appropriate SPME Fiber Coating –Effect of Analyte Molecular Weight and Polarity - [查看全部 (24)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ## 相關協定 - [Fast and Reliable Environmental Analysis of Aldehyde and Ketone Air Pollutants](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/fast-and-reliable) Aldehydes and ketones are ubiquitous air pollutants. Along with esters and ethers, they are major components of indoor air pollution and are therefore important for industrial hygiene applications. - [GC Analysis of Pyrazines in Peanut Butter](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/solid-phase-extraction/gc-analysis-of-pyrazines-in-peanut-butter) Separation of various pyrazines and sulfur compounds including carbon disulfide and dimethyl disulfide. - [Bisphenol A Analysis in Food by SPME](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/solid-phase-microextraction/analysis-of-bisphenol-a) Overcoated SPME fiber enables low-level BPA analysis in food via SPME-GC-MS/MS, meeting tighter migration limit regulations. - [Improved Determination of Volatile Organic Compounds in Water by SPME and GC/MS: ISO Standard 17943](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/drinking-water-testing/iso17943) High performance, reliability, and reproducibility of HS-SPME in combination with GC/MS for the determination of VOCs in water was proven in an interlaboratory trial. The new ISO 17943 using HS-SPME is an improvement on existing official methods for this determination in terms of sensitivity and selectivity. - [Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/analytical-chemistry/solid-phase-microextraction/standard-methods-gc-analysis-of-geosmin-and-slb) Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt - [查看全部 (17)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ### 查看更多文章與操作規範 輸入關鍵字 搜尋 ## 我們能為您提供什麼協助 如有任何疑問,請提交[客戶支援請求](https://www.sigmaaldrich.com/TW/zh/support/customer-support) 或聯絡我們的客戶服務團隊: 電郵至 custserv@sial.com 或致電 +1 (800) 244-1173 ## 額外支援 - [色譜圖搜尋](https://www.sigmaaldrich.com/chromatogram-search) 請使用「色譜圖搜尋」功能來鑑定樣本中的未知化合物。 - [計算機與應用程式](https://www.sigmaaldrich.com/TW/zh/support/calculators-and-apps) Web Toolbox - 用於分析化學、生命科學、化學合成和材料科學的科學研究工具和資源。 - [客戶支援請求](https://www.sigmaaldrich.com/TW/zh/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [常見問題](https://maestro.my.site.com/knowledgeportal/s/) 請瀏覽我們的「常見問題」,以獲取有關本公司產品與服務的常見問題解答。 * * * __精選文章__ [Selection Guide for Supelco SPME Fibers](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/selecting-spme-fibers) Recommended fibers based on analyte type and molecular weight; tips for SPME fiber selection for a variety of applications. [Supel™ BioSPME 96-Pin Devices](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/supel-biospme-96-pin-devices) Supel™ BioSPME 96-Pin devices enhance speed and simplicity for plasma protein binding studies and LCMS analyses. [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) Answers and tips relating to the application of the solid phase micro-extraction technique. [Rugged SPME Sampling](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) SPME fibers with CAR/PDMS and PDMS/DVB coatings on nitinol core assembly enhance fiber performance for solid phase microextraction. * * * ## 相關影片 Slide 1 of 4 [![Installing a SPME Fiber](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme-overview-installing-a-spme-fiber-poster.jpg "Installing a SPME Fiber")](https://www.sigmaaldrich.com) [Installing a SPME Fiber](https://www.sigmaaldrich.com) SPME is inexpensive and reusable. Beginners should consider using a manual SPME fiber and holder. The fiber assemblies are color-coded to signify the polymer coating on the fiber, and contain threads to screw into the holder barrel. [![觀看影片](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/spme-overview-installing-a-spme-fiber-poster.jpg "

SPME 概覽:安裝 SPME 光纖

")](https://www.sigmaaldrich.com) [SPME 概覽:安裝 SPME 光纖](https://www.sigmaaldrich.com) 固相微萃取(SPME)纖維可輕鬆安裝於 SPME 固定座中,亦可輕鬆更換。   [![使用 SPME 進行頭空間採樣是萃取揮發性有機化合物首選的方法。](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/headspace-sampling-of-vocs-with-spme-ms-poster.jpg "

SPME 概述:利用 SPME 進行揮發性有機化合物(VOC)的頭空間採樣

")](https://www.sigmaaldrich.com) [SPME 概述:利用 SPME 進行揮發性有機化合物(VOC)的頭空間採樣](https://www.sigmaaldrich.com) 使用 SPME 進行頭空間採樣是萃取揮發性有機化合物首選的方法。 [![固相微萃取(SPME)無溶劑萃取技術,是基於樣品與 SPME 纖維塗層之間的平衡原理。](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/video-posters/spme/headspace-sampling-of-vocs-with-spme-ms-poster.jpg "

SPME 概述(手動採樣):無溶劑樣品前處理

")](https://www.sigmaaldrich.com) [SPME 概述(手動採樣):無溶劑樣品前處理](https://www.sigmaaldrich.com) 固相微萃取(SPME)這種無溶劑萃取技術,是基於樣品與 SPME 纖維上的塗層之間的平衡。 頂部 __登入以繼續__ 若要繼續閱讀,請登入或建立帳戶。 登入__還沒有帳戶?__註冊 為便利客戶閱讀,此頁面中文以機器翻譯完成。雖然我們已盡力確保機器翻譯的準確性,但機器翻譯並非完美。如果您對機器翻譯的內容不滿意,請參考英文版本。 An unknown error has occured.