[跳轉至內容](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/TW/zh) 產品 購物車0 TWZH 產品 [登入 / 註冊](https://www.sigmaaldrich.com/oidc-sign-in) [訂單查詢](https://www.sigmaaldrich.com/TW/zh/order-lookup) [快速下訂](https://www.sigmaaldrich.com/TW/zh/quick-order) 購物車0 [首頁](https://www.sigmaaldrich.com/TW/zh)[應用](https://www.sigmaaldrich.com/TW/zh/applications)[基因組學](https://www.sigmaaldrich.com/TW/zh/applications/genomics)排序 # 排序 Slide 1 of 3 1 of 3 ![Sanger DNA 測序方法](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/genomics/sanger-sequencing-steps-process-diagram-01.jpg "Sanger Sequencing") ![Sanger DNA 測序方法](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/genomics/sanger-sequencing-steps-process-diagram-02.jpg "Sanger Sequencing") ![Sanger DNA 測序方法](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/genomics/sanger-sequencing-steps-process-diagram-03.jpg "Sanger Sequencing") 確定一條 DNA 或 RNA 鏈中核酸的組成和順序的能力對於理解現代遺傳學和眾多領域具有深遠的影響,而這些領域對於疾病研究和更好地理解所有生物都是必不可少的。核酸排序的能力不僅有助於破譯生物的遺傳代碼,還為研究人員提供了一個強大的工具,結合其他分子技術,可以輕鬆地操作 DNA 序列,並通過排序來驗證這些變化。由於編碼 DNA 的功能是為蛋白質提供編碼資訊,研究人員現在可以客製化設計包含大量功能元素的重組蛋白質,並廣泛用於回答同樣大量的重要研究問題。如需其他全基因組測序試劑和指南,請瀏覽[](https://www.sigmaaldrich.com)[Next-Generation Sequencing](https://www.sigmaaldrich.com/TW/zh/applications/genomics/next-gen-sequencing)資源. 。 * * * ## 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桑格測序 早期,分析化學方法能夠確定核酸組成。然而,Fred Sanger 及其同事開發了一些方法 - 最初使用放射性標籤消化片段和二維分餾 - 來提供一些最早的完整核酸序列。這個領域的進步持續了數十年,每一次的改進都為不斷增長的蛋白質和基因組測序文庫增添了新的內容。這些改進包括使用凝膠電泳(gel electrophoresis)按長度分離核苷酸,然後再使用毛細管電泳(capillary electrophoresis)。 ## DNA 測序方法 儘管 DNA 測序方法自此技術問世以來不斷改進,但現代 DNA 測序平台仍必須使用幾個基本元件。DNA 準備通常包括從宿主生物中分離和純化 DNA。其他關鍵組件,包括游離 DNA 基、DNA 引物、含有螢光標籤(終結基)的改良 DNA 基和 DNA 聚合酶會一起加入單一容器中。包含所有這些元件的容器經過一連串的加熱和冷卻步驟後,會產生一個相對於所需的全長 DNA 序列的小 DNA 序列庫,每個序列庫都以螢光末端標記的終止基為結尾。含有螢光末端標示核苷酸的新 DNA 鏈會按長度分開,通過毛細管,並按大小排列。然後用雷射激發每條鏈上的螢光基序,同時用攝影機捕捉信號。最後,電腦通常會將收集到的資訊組合為全長 DNA 序列。 [![文件搜尋](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/ecommerce/document-search.png "文件搜尋")](https://www.sigmaaldrich.com/documents-search) [尋找更多特定資訊?](https://www.sigmaaldrich.com/documents-search) 瀏覽我們的文件搜尋,以取得資料表、證書和技術文件。 [尋找文件](https://www.sigmaaldrich.com/documents-search) ## 相關文章 - [Validating CRISPR/Cas9-mediated Gene Editing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/advanced-gene-editing/validating-crispr-cas9-mediated-gene-editing) Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing. - [Ultrapure vs. DEPC Treated Water for Molecular Biology Applications](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/dna-and-rna-purification/nuclease-free-water) Understand the benefits of ultrapure water versus DEPC treatment in DNA and RNA experiments to ensure high purity and integrity without risks. - [Water for Next-Generation Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/next-gen-sequencing/water-for-next-generation-sequencing) Improve NGS performance and reliability by optimizing water quality at every step of the workflow, from fragmentation to cleaning to sequencing. - [Amino Acid Codon Wheel](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/amino-acid-codon-wheel) Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily. - [MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/multi-seq-sample-multiplexing-single-cell-analysis-sequencing) Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications. - [查看全部 (7)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ## 相關通訊協定 - [Sanger Sequencing Steps & Method](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/genomics/sequencing/sanger-sequencing) Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research. - [查看全部 (1)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search) ### 尋找更多文章和協議 輸入關鍵字 搜尋 ## How Can We Help 若有任何問題,請提交[客戶支援請求](https://www.sigmaaldrich.com/TW/zh/support/customer-support) 或與我們的客戶服務團隊討論: 電子郵件[[email protected]](mailto:[email protected]) 或致電 +1 (800) 244-1173 ## 額外支援 - [計算機與應用程式](https://www.sigmaaldrich.com/TW/zh/support/calculators-and-apps) Web Toolbox - 用於分析化學、生命科學、化學合成和材料科學的科學研究工具和資源。 - [Customer Support Request](https://www.sigmaaldrich.com/TW/zh/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __精選文章__ [Validating CRISPR/Cas9-mediated Gene Editing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/advanced-gene-editing/validating-crispr-cas9-mediated-gene-editing) Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing. [MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/multi-seq-sample-multiplexing-single-cell-analysis-sequencing) Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications. [Ultrapure vs. DEPC Treated Water for Molecular Biology Applications](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/dna-and-rna-purification/nuclease-free-water) Understand the benefits of ultrapure water versus DEPC treatment in DNA and RNA experiments to ensure high purity and integrity without risks. [Sequencing Primers](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/sequencing-primers) Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research. 頂部 __登入以繼續__ 若要繼續閱讀,請登入或建立帳戶。 登入__還沒有帳戶?__註冊 為便利客戶閱讀,此頁面中文以機器翻譯完成。雖然我們已盡力確保機器翻譯的準確性,但機器翻譯並非完美。如果您對機器翻譯的內容不滿意,請參考英文版本。 An unknown error has occured. - 中文 - ZH - English - EN [瞭解更多](https://www.sigmaaldrich.com)