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# 排序
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確定一條 DNA 或 RNA 鏈中核酸的組成和順序的能力對於理解現代遺傳學和眾多領域具有深遠的影響,而這些領域對於疾病研究和更好地理解所有生物都是必不可少的。核酸排序的能力不僅有助於破譯生物的遺傳代碼,還為研究人員提供了一個強大的工具,結合其他分子技術,可以輕鬆地操作 DNA 序列,並通過排序來驗證這些變化。由於編碼 DNA 的功能是為蛋白質提供編碼資訊,研究人員現在可以客製化設計包含大量功能元素的重組蛋白質,並廣泛用於回答同樣大量的重要研究問題。如需其他全基因組測序試劑和指南,請瀏覽[](https://www.sigmaaldrich.com)[Next-Generation Sequencing](https://www.sigmaaldrich.com/TW/zh/applications/genomics/next-gen-sequencing)資源.
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## 相關產品
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Sigma-Aldrich
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T4415
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Tris-硼酸-EDTA缓冲溶液](https://www.sigmaaldrich.com/TW/zh/product/sigma/t4415)
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Roche
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FPCRN-RO
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FastStart ™ Taq DNA 聚合酶,dNTPack](https://www.sigmaaldrich.com/TW/zh/product/roche/fpcrnro)
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Sigma-Aldrich
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P5475
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C-CMV-24 Sequencing Primer](https://www.sigmaaldrich.com/TW/zh/product/sigma/p5475)
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Sigma-Aldrich
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HSANGERV
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桑格全基因组 慢病毒 CRISPR 文库](https://www.sigmaaldrich.com/TW/zh/product/sigma/hsangerv)
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Sigma-Aldrich
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HSANGERG
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桑格全基因组 慢病毒 CRISPR 文库](https://www.sigmaaldrich.com/TW/zh/product/sigma/hsangerg)
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Sigma-Aldrich
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MSANGERG
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桑格全基因组 慢病毒 CRISPR 文库](https://www.sigmaaldrich.com/TW/zh/product/sigma/msangerg)
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Sigma-Aldrich
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MSANGERV
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桑格全基因组 慢病毒 CRISPR 文库](https://www.sigmaaldrich.com/TW/zh/product/sigma/msangerv)
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NA1020
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GenElute™ PCR 纯化试剂盒](https://www.sigmaaldrich.com/TW/zh/product/sigma/na1020)
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S3938
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SeqSaver™ Sequencing Premix Dilution Buffer](https://www.sigmaaldrich.com/TW/zh/product/sigma/s3938)
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UC9604
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UltraClear™ 测序反应纯化试剂盒](https://www.sigmaaldrich.com/TW/zh/product/sigma/uc9604)
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D1947
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Diffinity RapidTip®](https://www.sigmaaldrich.com/TW/zh/product/sigma/d1947)
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概述
相關文章與協議
支援
## 桑格測序
早期,分析化學方法能夠確定核酸組成。然而,Fred Sanger 及其同事開發了一些方法 - 最初使用放射性標籤消化片段和二維分餾 - 來提供一些最早的完整核酸序列。這個領域的進步持續了數十年,每一次的改進都為不斷增長的蛋白質和基因組測序文庫增添了新的內容。這些改進包括使用凝膠電泳(gel electrophoresis)按長度分離核苷酸,然後再使用毛細管電泳(capillary electrophoresis)。
## DNA 測序方法
儘管 DNA 測序方法自此技術問世以來不斷改進,但現代 DNA 測序平台仍必須使用幾個基本元件。DNA 準備通常包括從宿主生物中分離和純化 DNA。其他關鍵組件,包括游離 DNA 基、DNA 引物、含有螢光標籤(終結基)的改良 DNA 基和 DNA 聚合酶會一起加入單一容器中。包含所有這些元件的容器經過一連串的加熱和冷卻步驟後,會產生一個相對於所需的全長 DNA 序列的小 DNA 序列庫,每個序列庫都以螢光末端標記的終止基為結尾。含有螢光末端標示核苷酸的新 DNA 鏈會按長度分開,通過毛細管,並按大小排列。然後用雷射激發每條鏈上的螢光基序,同時用攝影機捕捉信號。最後,電腦通常會將收集到的資訊組合為全長 DNA 序列。
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## 相關文章
- [Validating CRISPR/Cas9-mediated Gene Editing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/advanced-gene-editing/validating-crispr-cas9-mediated-gene-editing)
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
- [Ultrapure vs. DEPC Treated Water for Molecular Biology Applications](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/dna-and-rna-purification/nuclease-free-water)
Understand the benefits of ultrapure water versus DEPC treatment in DNA and RNA experiments to ensure high purity and integrity without risks.
- [Water for Next-Generation Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/next-gen-sequencing/water-for-next-generation-sequencing)
Improve NGS performance and reliability by optimizing water quality at every step of the workflow, from fragmentation to cleaning to sequencing.
- [Amino Acid Codon Wheel](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/amino-acid-codon-wheel)
Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.
- [MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/multi-seq-sample-multiplexing-single-cell-analysis-sequencing)
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
- [查看全部 (7)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search)
## 相關通訊協定
- [Sanger Sequencing Steps & Method](https://www.sigmaaldrich.com/TW/en/technical-documents/protocol/genomics/sequencing/sanger-sequencing)
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
- [查看全部 (1)](https://www.sigmaaldrich.com/TW/zh/search/facet-search?focus=sitecontent&term=facet-search)
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__精選文章__
[Validating CRISPR/Cas9-mediated Gene Editing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/advanced-gene-editing/validating-crispr-cas9-mediated-gene-editing)
Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.
[MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/multi-seq-sample-multiplexing-single-cell-analysis-sequencing)
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
[Ultrapure vs. DEPC Treated Water for Molecular Biology Applications](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/dna-and-rna-purification/nuclease-free-water)
Understand the benefits of ultrapure water versus DEPC treatment in DNA and RNA experiments to ensure high purity and integrity without risks.
[Sequencing Primers](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/genomics/sequencing/sequencing-primers)
Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.
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