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Merck

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11644793001

Roche

细胞毒性检测试剂盒(LDH)

suitable for protein quantification, suitable for cell analysis, detection, sufficient for ≤2,000 tests

同義詞:

LDH

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UNSPSC Code:
12352200
NACRES:
NA.54
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usage

sufficient for ≤2,000 tests

packaging

pkg of 1 kit

manufacturer/tradename

Roche

technique(s)

protein quantification: suitable

λmax

490 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

−20°C

General description

作为[51Cr]以及[3H]-胸苷释放检测的一种非放射性替代方法。
比色检测用于细胞死亡和细胞裂解的定量,基于对从受损细胞胞液释放至上清中的乳酸脱氢酶(LDH)进行测定。

Application

细胞毒性检测试剂(LDH)是一种基于96孔板形式对来自受损细胞释放的LDH活性进行快速而简单测量的方法,用于对细胞毒性/细胞裂解进行量化。因此,当对细胞质膜的损伤发生时,该试剂盒可用于许多不同体外细胞系统。例如:
  • 细胞介导的细胞毒性检测和定量
  • 调解因子介导的细胞裂解测定
  • 测定化合物在环境和医学研究中细胞毒性潜能,应用于食品、化妆品及制药行业[1][2][3]
  • 测定生物反应器中的细胞死亡
  • 检测在骨组织工程中用于骨细胞生长的多种支架的生物兼容性[4][5]

Packaging

1个试剂盒包含2种组分。

Preparation Note

工作溶液:瓶1催化剂:
用1 ml双蒸水将冻干酶重悬10分钟并充分混匀。

瓶2染料溶液:
即用型溶液。

反应混合物
用于100次检测:在使用前不久,将瓶1中的250 μl与瓶2中的11.25 ml的进行混合。
用于400次检测:在使用前不久,将全部体积的催化剂(1 ml)加入至全部体积的
染料溶液(45 ml)并充分混匀。

注意:反应混合物不应当进行储备;在立即使用前进行制备。
储备条件(工作溶液):2至8°C
冻干酶可在2至8°C稳定
重悬的催化剂溶液可在2至8°C稳定储存四周
一旦解冻,染料溶液可在2至8°C稳定储存数周。

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本產品
CYTODET-ROTOX7TOX8
technique(s)

protein quantification: suitable

technique(s)

protein quantification: suitable

technique(s)

protein quantification: suitable

technique(s)

protein quantification: suitable

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric, fluorometric

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

packaging

pkg of 1 kit

packaging

kit of 1 (4 components)

packaging

pkg of 1 kit

packaging

pkg of 1 kit


僅套裝組件

產品號碼
描述

  • Catalyst (Diaphorase/NAD+ mixture)

  • Dye Solution (INT and sodium lactate)

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

存儲類別/等級

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash



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分析證明 (COA)

Lot/Batch Number

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文章

品質控制指導方針,以維持高品質、經驗證且無污染的細胞培養物。免費下載 ECACC 手冊。

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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