usage
sufficient for ≤2,000 tests
packaging
pkg of 1 kit
manufacturer/tradename
Roche
technique(s)
protein quantification: suitable
λmax
490 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
−20°C
General description
比色检测用于细胞死亡和细胞裂解的定量,基于对从受损细胞胞液释放至上清中的乳酸脱氢酶(LDH)进行测定。
Application
Packaging
Preparation Note
用1 ml双蒸水将冻干酶重悬10分钟并充分混匀。
瓶2染料溶液:
即用型溶液。
反应混合物
用于100次检测:在使用前不久,将瓶1中的250 μl与瓶2中的11.25 ml的进行混合。
用于400次检测:在使用前不久,将全部体积的催化剂(1 ml)加入至全部体积的
染料溶液(45 ml)并充分混匀。
注意:反应混合物不应当进行储备;在立即使用前进行制备。
储备条件(工作溶液):2至8°C
冻干酶可在2至8°C稳定
重悬的催化剂溶液可在2至8°C稳定储存四周
一旦解冻,染料溶液可在2至8°C稳定储存数周。
1 of 1
本產品 | |||
|---|---|---|---|
| technique(s) protein quantification: suitable | technique(s) protein quantification: suitable | technique(s) protein quantification: suitable | technique(s) protein quantification: suitable |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric, fluorometric |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| packaging pkg of 1 kit | packaging kit of 1 (4 components) | packaging pkg of 1 kit | packaging pkg of 1 kit |
僅套裝組件
- Catalyst (Diaphorase/NAD+ mixture)
- Dye Solution (INT and sodium lactate)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存儲類別/等級
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
文章
品質控制指導方針,以維持高品質、經驗證且無污染的細胞培養物。免費下載 ECACC 手冊。
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
相關內容
Cytotoxicity Detection Kit (LDH) Protocol & Troubleshooting




