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A3912

牛血清白蛋白来源于牛血清

Name: 牛血清白蛋白 来源于牛血清
Brand: SIGMA

heat shock fraction, pH 5.2, ≥96%

同義詞:

BSA, 牛白蛋白, 牛血清白蛋白

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CAS 編號:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96%

Form:

lyophilized powder

Technique(s):

blood typing: suitable, microbiological culture: suitable

Impurities:

BT and VSV virus, tested

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屬性

biological source

bovine

Quality Segment

200

assay

≥96%

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable, microbiological culture: suitable

impurities

BT and VSV virus, tested

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

描述

General description

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry.[1] Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

Application

Bovine Serum Albumin (BSA) has been used:

to study its adsorption on a planar poly(acrylic acid) (PAA) brush layer through fixed-angle optical reflectometry
to measure its adsorption on a variety of thin films by means of total internal reflection spectroscopy
as a constituent of Krebs-Ringer bicarbonate buffer, which acts as a perfusate during placental perfusion

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。更多的纯化步骤包括结晶或木炭过滤。

采用热休克分级分离法制备

Analysis Note

残余物主要是球蛋白。

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本產品	A3059	A4503	A9056
$牛血清白蛋白来源于牛血清 heat shock fraction, pH 5.2, ≥96%$ Sigma-Aldrich A3912 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%$ Sigma-Aldrich A3059 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 cold ethanol fraction, pH 5.2, ≥96%$ Sigma-Aldrich A4503 牛血清白蛋白来源于牛血清	$牛血清白蛋白来源于牛血清 heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)$ Sigma-Aldrich A9056 牛血清白蛋白来源于牛血清
description heat shock fraction, pH 5.2, ≥96%	description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%	description cold ethanol fraction, pH 5.2, ≥96%	description heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)
origin USA origin	origin USA origin	origin USA origin	origin USA origin
assay ≥96%	assay ≥98%	assay ≥96%	assay ≥96% (agarose gel electrophoresis)
biological source bovine	biological source bovine	biological source bovine	biological source bovine
technique(s) blood typing: suitable, microbiological culture: suitable	technique(s) ELISA: suitable, western blot: suitable	technique(s) ELISA: suitable, blood typing: suitable, microbiological culture: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 300	Quality Level 300	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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存儲類別/等級

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c