Although not validated, many users choose to aliquot the Accumax into single use vials after the initial thaw to store frozen at -20 °C until the expiration date.
sterility
sterile-filtered
Quality Level
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
Application
Accumax已经证明能够在杂交瘤、CHO、BHK、293、COS和Sf9细胞的悬浮培养物中对成簇细胞进行有效解离。 该溶液在解离细胞计数、病毒转染测定、细胞分选、流式细胞术和生物反应器放大方面具有极佳的表现。 它还被用于从聚集的细胞培养物中获得单细胞悬浮液，以实现精确的细胞计数、细胞与原发组织的分离、以及细胞与标准组织培养塑料器皿和粘附涂层塑料器皿的常规分离。
TM = [“Accumax”]溶液已用于
- 从上皮细胞的细胞外基质(ECM)解离单壁碳纳米管
- 在传代前解离视网膜色素层细胞
- 从胰腺上解离胰岛细胞
Biochem/physiol Actions
Accumax是一种细胞分离溶液，由蛋白水解酶和胶原溶解酶组成。 每瓶含有100 mL溶于杜氏′PBS的1x Accumax酶。Accumax中没有发现哺乳动物或细菌衍生产物。
Features and Benefits
用于体外 细胞应用的即用型无菌液体
Preparation Note
在杜氏′PBS（0.2g/L KCl、0.2g/L KH2PO4、8g/L NaCl和1.15g/L Na2HPO4）中进行制备。 有关产品使用信息，请参阅网站上的产品数据表。
本产品以无菌即用型冷冻液的形式提供。 Accumax应在使用前在37°C解冻，并以等体积加入至集聚细胞样品中。 在37°C下孵育5-10分钟，然后进行正常计数细胞。
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
Disclaimer
Accumax在-20°C下储存可保持稳定。解冻后，产品可在4°C下储存长达2个月。
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本產品 | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form solution | form liquid | form liquid | form solution |
| concentration 1 × | concentration 400-600 units/mL | concentration - | concentration 1 × |
| pH 6.8-7.8 | pH 6.8-7.8 | pH - | pH 7.0-9.0 |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile; sterile-filtered |
| storage temp. −20°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. −20°C |
存儲類別/等級
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
文章
肺有機體是研究人類肺部發育和呼吸系統疾病的寶貴 3D 模型。3dGRO™ 分化方案通過 4 個步驟從人類 iPSCs 生成器官組織。
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
使用 MULTI-seq 脂質修飾寡聚體、PCR Assays 和 Sequencing 應用的協議和故障排除指南。
相關內容
Datasheet
Natalja Funk et al.
Scientific reports, 9(1), 4515-4515 (2019-03-16)
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role of LRRK2 mutations remain uncertain. Here, we investigated the role of LRRK2 in intracellular signal
Benjamin T Dodsworth et al.
Scientific reports, 10(1), 10542-10542 (2020-07-01)
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
Geng Z, et al.
PLoS ONE, 12(3), e0173575-e0173575 (2017)
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After thawing Accumax, can I make aliquots and freeze them?
1 answer-
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Is your accumax gmp grade and made in a gmp compliant facility and is animal free?
1 answer-
This Accumax™ solution does not contain mammalian- or bacterial-derived products. It is not a GMP grade. This product is assigned a quality level of 200. Products with quality level of 200 are not suitable for GMP. Products with quality levels 400 or higher are suitable for GMP.
Currently, there is no GMP grade product available.Please see the quality brochure below for more information:
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What is the size of the bottle in size specifications (inches, centimeters)?
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See below for the 100 mL and 500 mL bottle dimensions:
100 mL
Height: 104.1 mm
Height w/cap: 108.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 37.3 mm500 mL
Height: 173.2 mm
Height w/cap: 177.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 38 mmHelpful?
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What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
1 answer-
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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