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Merck

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F1804

单克隆抗-FLAG® M2 小鼠抗

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

ANTI-FLAG® M2 antibody, Mouse monoclonal

同義詞:

flag抗体, 抗 ddddk, 抗 dykddddk

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關於此項目

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
Citations:
9676
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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1.0-1.2 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

General description

小鼠抗-FLAG M2抗体,可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。

纯化方法 - 亲和标签纯化

Immunogen

FLAG;肽序列DYKDDDDK

Application

用于通过免疫印迹、免疫沉淀(IP)[1]、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于哺乳动物、植物和细菌表达系统中FLAG融合蛋白的单条带检测。

蛋白质免疫印迹和EIA

更多产品信息请访问我们的FLAG®应用页面。

Biochem/physiol Actions

结合位点:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

在磷酸盐缓冲液中含有50%甘油。

Preparation Note

在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液(TBS),pH 8.0

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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本產品
F2426F4049A9594
antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

-

conjugate

FITC conjugate

conjugate

CY3 conjugate

biological source

mouse

biological source

-

biological source

mouse

biological source

mouse

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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flash_point_f

Not applicable

flash_point_c

Not applicable

存儲類別/等級

10 - Combustible liquids



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分析證明 (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. Can this Antibody be used for Flow cytometry?

    1 answer
    1. While this antibody hasn't been validated for flow cytometry, independent laboratories have published its use for this application. For example:
      https://www.nature.com/articles/s42003-020-1072-4.pdf

      Helpful?

  2. Any protocol available for using the Anti-Flag M2 (F1804) in a ChIP-Seq assay? And, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?

    1 answer
  3. Is this Ab suitable for ELISA?

    1 answer
    1. This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.

      Helpful?

  4. Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci

    1 answer
    1. Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
      https://www.nature.com/articles/s41467-019-12187-5.pdf

      Helpful?

  5. モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。

    1 answer
    1. An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf

      Helpful?

Reviews

Active Filters

  1. Technion-Israel Institute of Technology
    • Review 1
    • Votes 23
    5 out of 5 stars.

    Perfection

    Perfect for WB and IF, very specific. Stable for many years.

    Helpful?

  2. 2 Ratings-only reviews