Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
F2442
胎牛血清
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
同義詞:
FBS, FCS, 血清
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Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
暫時無法取得訂價和供貨情況
biological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
胎牛血清（FBS）是一种由低、高分子量生物分子组成的复杂混合物，具有最佳的生长促进和抑制功能。FBS中含有如生长因子、蛋白质、维生素、微量元素、激素等多种分子。这些分子对于细胞的生长和维持至关重要。它是细胞培养基中的一种常见补剂。FBS来自于小牛血。它不含有纤维蛋白和凝血因子。FBS的热灭活可阻断补体系统。来源于凝结血的FBS是真核细胞，尤其是哺乳动物细胞培养中最广泛使用的未确定补充剂。[1]它通常与在1950年代和1960年代开发的经典培养基一起使用。这些包括多种常用培养基，如Minimum Essential培养基（MEM）、[2]Dulbecco′s改良Eagles培养基（DME，DMEM）、Iscove′s改良Eagles培养基（IMDM）、[3]M199[4]以及Roswell Park Memorial Institute培养基RPMI-1640。[2]
Application
Biochem/physiol Actions
FBS仍然是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子，并通过难以在无血清培养基（SFM）系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。
Analysis Note
- 内毒素和血红蛋白测试
- 检测了是否存在细菌、病毒和支原体
- 在无菌条件下用0.1微米膜过滤三次
Other Notes
对是否存在细菌、病毒、支原体、内毒素以及血红蛋白进行了检测。在无菌条件下用0.1微米膜过滤三次。
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本產品 | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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存儲類別/等級
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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is heat inactivation necessary?
1 answer-
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How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 answer-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.Helpful?
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is this Heat Inactivate FBS?
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This product has not been heat inactivated.
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can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
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This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317Helpful?
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Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 answer-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/Helpful?
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What is the level of growth factors in Fetal Bovine Serum, Product F2442?
1 answer-
Sigma Aldrich does not determine the levels of growth factors (such as TGF beta, VEGF, EGF, PDGF, or FGF) for serum with reduced levels of growth factors and plasma derived serum.
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Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?
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We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.
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What is the difference between Fetal Bovine Serum F2442 and 12103C?
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Product No. 12103C meets most of the specifications of F2442. Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
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FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Are all Fetal Bovine Serum (FBS) products heat inactivated?
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Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
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