Reliable cell culture product that gets frequent use
T3924
胰蛋白酶-EDTA 溶液
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Trypsin-EDTA solution
1 of 2 reviewers received a sample product or took part in a promotion
同義詞:
Cell dissociation enzyme
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| 包裝尺寸 | 庫存單位 | 供貨能力 | 價格 |
|---|
關於此項目
Biological source:
Porcine
Concentration:
1 ×
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biological source
Porcine
Quality Level
sterility
sterile; sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-8.0
shipped in
dry ice
storage temp.
−20°C
Application
该产品的典型用途是使黏附细胞从培养表面脱落。 使细胞从附着面脱落所需的胰蛋白酶浓度主要取决于细胞类型和培养阶段。
适合用于制备测序用单细胞悬液。
适合用于制备测序用单细胞悬液。
Biochem/physiol Actions
胰蛋白酶可切割赖氨酸和精氨酸残基的C末端肽段。 如果酸性残基位于切割位点的任意一侧，则该反应的水解速度减慢，并且如果脯氨酸残基位于切割位点的羧基侧，则水解停止。 胰蛋白酶活性的最佳pH条件为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯和酰胺键。 将EDTA添加到胰蛋白酶溶液中作为螯合剂，用于中和钙离子和镁离子以防止其抑制胰蛋白酶活性。 去除这些离子会增加酶活性。
丝氨酸蛋白酶抑制剂（包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等）会抑制胰蛋白酶。
丝氨酸蛋白酶抑制剂（包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等）会抑制胰蛋白酶。
Preparation Note
本产品含有酚红。 由于在干冰上运输，包装中可能累积大量二氧化碳。 这些CO2可能会进入溶液并略微降低pH值，使溶液呈橙色而不是粉红色。 橙色溶液仍然可以使用，或者可以用氢氧化钠调节pH。 使用高浓度胰蛋白酶长时间孵育细胞，会破坏细胞膜并杀死细胞。 溶解胰蛋白酶或稀释浓缩胰蛋白酶溶液时，应使用不含Ca2+ 或Mg2+的缓冲盐溶液。
Other Notes
胰蛋白酶是由含223个氨基酸残基的单链多肽组成的，它通过切割胰蛋白酶原的Lys-1le肽键以去除N-末端六肽而获得。 氨基酸序列通过6个二硫键交联。 这是天然形式的胰蛋白酶——β-胰蛋白酶。 β-胰蛋白酶可以自溶，在Lys-Ser残基处断裂，产生α-胰蛋白酶。 胰蛋白酶是丝氨酸蛋白酶家族的成员。
Disclaimer
本品应置于-10至-40°C环境冷冻储存。避免反复冻融。
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本產品 | |||
|---|---|---|---|
| biological source Porcine | biological source Porcine | biological source Porcine | biological source Porcine pancreas |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form solution | form solution | form solution | form solution |
| concentration 1 × | concentration 0.25% | concentration 10 × | concentration 1 × |
| pH 7.0-9.0 | pH 7.0-7.6 | pH 7.0-7.6 | pH 7.0-7.6 |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt - |
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存儲類別/等級
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
條款
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
使用人類支氣管上皮細胞系的技術資訊，包括解凍、亞培養和冷凍保存。
文章
Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.
Shiva Hamidian Jahromi et al.
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Tumor necrosis factor alpha (TNF-α) induced protein 6 is a major anti-inflammatory mediator released by activated mesenchymal stromal cells (MSCs). Neonatal MSCs are considered more metabolically active than cells derived from adult tissues, and potentially less heterogeneous. We hypothesized that
Katrin Kleinmanns et al.
EBioMedicine, 56, 102783-102783 (2020-05-27)
The completeness of resection is a key prognostic indicator in patients with ovarian cancer, and the application of tumour-targeted fluorescence image-guided surgery (FIGS) has led to improved detection of peritoneal metastases during cytoreductive surgery. CD24 is highly expressed in ovarian
Ashraf Hassanpour et al.
Stem cell research & therapy, 9(1), 252-252 (2018-09-28)
The increasing number of patients with ovarian insufficiency due to autoimmune disorders, genetic predisposition, or iatrogenic effects of treatment such as cancer therapies necessitates an urgent measure to find a safe and transplantable alternative ovary. A bioengineered ovary is one