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Merck

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W1754

PCR Reagent, suitable for PCR

同義詞:

H2O

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關於此項目

線性公式:
H2O
CAS 編號:
分子量::
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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產品名稱

水, PCR Reagent

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected, RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

General description

对 PCR 级水进行无菌过滤。它不含核酸外切酶(dna 酶、rna 酶)和核酸内切酶(切口酶),也不含核酸污染。

Application

适用于聚合酶链式反应 (PCR)
水已用于:

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分,以扩增真菌(杂色云芝)DNA的产物
  • 作为稀释剂和反应混合物的组分,以扩增cDNA[1]
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积[1]

Other Notes

便于将水产品规格与 水规格表进行比较

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本產品
952849801-OP4.86505
technique(s)

PCR: suitable

technique(s)

FISH: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

300

form

liquid

form

liquid

form

liquid

form

liquid

pH

5-7

pH

5-7

pH

-

pH

-

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

vapor density

<1 (vs air)

vapor density

-

vapor density

-

vapor density

-


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存儲類別/等級

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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條款

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

檢視所有通訊協定

Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)