CRISPR Cas9 蛋白與基因編輯
THE ULTIMATE SUITE OF CRISPR CAS9 PROTEIN ESSENTIALS AND SERVICES
基因組工程的進展讓研究人員得以實現前所未有的突破，從治療遺傳疾病到開發抗病作物。我們可靠的 CRISPR Cas9 蛋白質必需品和工具為從台式到更大規模的 CRISPR 基因編輯研究的每個步驟提供完整的支援。使用鋅指核酸酶靶向單一基因。從兩種不同的 PURedit® Cas9 胞嘧啶碱基編輯套件中選擇 C 到 T 的取代，優化到獨特的編輯視窗，以進行高難度的編輯。
從 CRISPR Cas9 蛋白、合成導向 RNA 到用於 CRISPR 基礎篩選的慢病毒產品，我們為您的 CRISPR 基礎基因編輯研究的每個步驟提供了終極的產品套件。
Sigma-Aldrich®先進基因組學產品組合提供終極的基因組工程工具和服務套件，釋放您的研究潛力。無論您是要敲除、敲入、敲除或過度表達您的目標，我們全面的 CRISPR 基因編輯工具和服務套件將帶領您的研究超越工作台。
產品
瞭解 PURedit® Cas9 胱氨酸基底編輯套件如何立即提供正確的編輯。
Best in Class Cas9種蛋白質，包括PURedit® Cas9 胞嘧啶碱基编辑蛋白试剂盒
Sigma-Aldrich® 以多种形式（质粒、慢病毒、冻干蛋白）提供多种 Cas9 变体（野生型、特异性增强型、镍酶、GFP 融合型、催化不活跃型）。我們最新推出的 PURedit® Cas9 胞嘧啶碱基編輯蛋白試劑盒，可使用我們的專家設計的兩種酵素進行定義的胞嘧啶碱基編輯，方便 RNP 傳遞，專為成功而設計。
Guaranteed CRISPR gRNA
Every SygRNA® ；合成的單一導向 RNA (sgRNA)、crRNA 和 tracrRNA 都是使用我們的專利製程和試劑，以最高品質的材料合成而成。無論您的目標、細胞類型或應用是什麼，我們都能保證編輯活性，並提供業界最全面的保證。利用我們免費的客製化 CRISPR 設計工具應用程式，您可以為所有類型的 CRISPR 應用設計自己的指南，包括使用我們的 PURedit® Cas9 Precision 和 Flexible Cytosine Base Editors 進行胞嘧啶碱基編輯。
CRISPR抑制和激活（CRISPRi和CRISPRa）
使用 Sigma-Aldrich® CRISPR 激活和 CRISPR 抑制文庫進行基因敲除和過度表達實驗，可以更深入地瞭解複雜的生物網絡。匯集的 CRISPRi 和 SAM CRISPRa 慢病毒文庫可現成提供，也可根據您的特定需求定制。
具有 10x Genomics 兼容性的 CRISPR 篩選
Sigma-Aldrich® 為 CRISPR 單細胞篩選提供唯一的 10x Genomics 認證兼容慢病毒池。我們的優化、客製化慢病毒載體利用特徵條碼技術與 Chromium 單細胞基因表達 v3 和 v3.1 (Next GEM) 解決方案完全相容。
相關資源
- Brochure: Molecular Essentials Guide
Learn how our comprehensive molecular tools and reagents provide precise, ready-to-use solutions for your workflows.
- Article: CRISPR Cas9 Proteins
優質的 Cas9 蛋白可確保高效的基因編輯，並提高特異性和活性，滿足多樣化的 CRISPR 試劑需求。
- Article: CRISPR Cas9 Protein Product Selection Guide
A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.
- CRISPR Use License Agreement
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- Article: PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity.
- eBook: Gene Editing 101
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: Blue-White Screening & Protocols for Colony Selection
Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.
- Article: Introduction to Cell Transfection
An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.
- Protocol: Western Blotting
Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.
- Article: CRISPRi & CRISPRa Genetic Screening
Explore optimized CRISPR libraries for gene modulation experiments, offering off-the-shelf or customizable options.
- Article: Single-Cell Resolution Discovery
與 10x Genomics 合作的 CRISPR 慢病毒篩選文庫，為集合篩選提供強大的研究工具。
- Flyer: Determining Which Screening Option is Right for Your Experiment
Designing a screening experiment can be daunting with all the technologies and formats available. With the largest portfolio of screening tools on the market, we can help ensure you have the right tools at the right scale to meet your goals.
- Flyer: CRISPRi Whole Genome and Non-Coding Libraries
Lentiviral libraries and pools to take your research beyond the bench.
- Flyer: Whole Genome SAM CRISPRa Libraries
A complete kit for genome scale activation.
- Flyer: CRISPR Dual Guide Lentiviral Vectors
Double the editing power with dual gRNAs and integrated 10x genomics compatibility for single-cell analysis.
網路工具
- PCR 母液計算機
使用此線上 PCR 母液混合計算機，執行大規模 PCR 反應的計算，並確保放大反應的成功。
- CRISPR Design Tool
Design your guide RNA for standard Cas9 editing and PURedit® Cas9 Cytosine Base Editor Proteins.
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