Duolink® 近接分析法
Duolink® Proximity Ligation Assays (PLA) 能夠研究內源蛋白質-蛋白質互作 (PPI) 和轉譯後修飾，為單一事件提供螢光信號，可透過顯微鏡或流式細胞計顯示訊號。為了研究蛋白質與蛋白質之間的互動，Duolink® PLA 技術使用一對以寡核苷酸標記的抗體，當這對抗體存在於 40 nm 之內，在加入標記探針後進行滾圓擴增，產生特定的螢光信號。
Duolink®PLA有四種不同的顏色，適用於流式細胞計及顯微鏡，可顯示每個細胞中多種蛋白質與蛋白質之間的相互作用。利用 Duolink® PLA 技術的優點與擴大效能，發掘您的研究機會：
- 檢視蛋白質互作 - 包括穩定和瞬間的互作
- 檢視轉譯後修飾
- 檢測內源蛋白 - 不需要過度表達或基因操作
- 高特異性 - 使用兩種抗體/探針可消除假陽性
- 不需要特殊設備 - 可使用標準的免疫螢光和流式細胞計方法
產品
Duolink® PLA 產品
- Duolink® PLA Starter Kits 包含進行螢光實驗和分析多達 30 個樣本所需的全部試劑。
- Duolink® Probemaker Kits 可將 PLA 寡聚體 (PLUS 或 MINUS) 與任何抗體結合。
- Duolink® PLA Multicolor Kits 可讓顯微鏡觀察多種 PPI。
- Duolink® flowPLA Detection Kits 可讓流式細胞計應用使用四種顏色：綠色、橙色、紅色和遠紅色。
- Duolink® PLA Brightfield Detection 含有辣根過氧化酶 (HRP) 標記的寡核苷酸，可與光學顯微鏡搭配使用。
相關資源
- Application Note: An Introduction to Duolink® PLA
Duolink® PLA protein detection technology is based on in situ proximity ligation assay (PLA), and allows for the detection, visualization, and quantification of protein expression, protein-protein interactions, and post-translational modifications, in unmodified cells and tissue samples within a matter of hours.
- Creating PLA® Probes Protocol
Duolink®试剂盒使用原位PLA®技术（一种邻位连接检测技术）准确、客观地量化单个蛋白质，以及这些蛋白在未修饰细胞和组织中的互作和修饰。
- Article: Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
- Article: Duolink® PLA Flow Cytometry Kits
Duolink® PLA kit enhances flow cytometry for detecting protein interactions accurately.
- Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
- Duolink® PLA Fluorescence Protocol
This protocol describes the use of Duolink® PLA reagents for the immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
- Application Note: How to Optimize the Duolink® Proximity Ligation Assay
To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol.
- Brochure: Amplified Detection Duolink® Proximity Ligation Assay (PLA)
Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.
- Application Note: Duolink® PLA Probemaker Guide
This guide describes the use of the Duolink® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink® PLA experiments.
- Flyer: Duolink® Flow Cytometry
Traditional flow cytometry has been limited in the ability to detect protein interactions and low abundant proteins events — until now.
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