[跳轉至內容](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/TW/zh) 產品 購物車0 TWZH 產品 產品應用服務資源支援 [登入 / 註冊](https://www.sigmaaldrich.com/oidc-sign-in) [訂單查詢](https://www.sigmaaldrich.com/TW/zh/order-lookup) [快速下訂](https://www.sigmaaldrich.com/TW/zh/quick-order) 購物車0 [Home](https://www.sigmaaldrich.com/TW/en)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/TW/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)Manual SPME Sampling # Manual SPME Sampling [Learn More About SPME](https://www.sigmaaldrich.com/TW/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction) Beginners should consider using a manual SPME fiber and holder. The manual system allows the analyst to optimize the extraction process by evaluating different fibers and extraction conditions in a consistent manner. Begin the extraction process by placing the sample into the vial puck on the hotplate. The vial puck allows users to easily index the vial to begin sample agitation. Insert the holder into the sampling stand, passing the needle through the septum that seals the sample vial. Depress the plunger down to expose the fiber to the sample liquid or headspace above the sample. Start the timing of exposure of the SPME fiber for reproducible extractions. After a predetermined time, the SPME fiber is retracted into the protective needle and transferred to the GC for analysis. Inject the SPME fiber into the GC injection port, where the collected organics are quickly transferred to the GC column by heating the fiber. After the thermal transfer of analytes is completed, the fiber is removed and inserted in the next sample vial for extraction. __Related Articles__ - [Biodiesel](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/biodiesel) - [Automated Solid Phase Microextraction (SPME) Sampling](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/automated-spme-sampling) - [Bioanalysis with SPME](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/bioanalysis-with-spme) - [BioSPME for Plasma Protein Binding Assay: Details of Method Development and Optimization](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/biospme-plasma-protein-binding-assay) - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) - [GC/MS/MS Analysis of BPA in Energy Drink](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/gc-ms-ms-analysis-of-bisphenol-a-bpa-in-a-fruit-flavored-energy-drink-cla010218a) - [Rugged SPME Sampling](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) - [SPME Fiber Performance Tips for Volatiles & Semi-Volatiles](https://www.sigmaaldrich.com/TW/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/performance-tips) - [View More](https://www.sigmaaldrich.com/TW/en/search/facet-search?focus=sitecontent&term=facet-search) 頂部 __登入以繼續__ 若要繼續閱讀，請登入或建立帳戶。 登入__還沒有帳戶？__註冊 支援[客戶支援](https://www.sigmaaldrich.com/TW/zh/support/customer-support)[聯絡我們](https://www.sigmaaldrich.com/TW/zh/collections/offices)[常見問題](https://maestro.my.site.com/knowledgeportal/s/)[安全資料表 (SDS)](https://www.sigmaaldrich.com/TW/zh/documents-search?tab=sds)[證書 (COA/COO)](https://www.sigmaaldrich.com/TW/zh/documents-search?tab=coa)[品質與法規](https://www.sigmaaldrich.com/TW/zh/life-science/quality-and-regulatory-management)[計算機與應用程式](https://www.sigmaaldrich.com/TW/zh/support/calculators-and-apps)[網路研討會](https://www.sigmaaldrich.com/TW/zh/collections/webinars) 訂單[訂單查詢](https://www.sigmaaldrich.com/TW/zh/order-lookup)[快速訂購](https://www.sigmaaldrich.com/TW/zh/quick-order)[客製化產品](https://www.sigmaaldrich.com/TW/zh/services/custom-products)[電子商務解決方案](https://www.sigmaaldrich.com/TW/zh/life-science/ecommerce/ecommerce-solutions) 公司簡介[關於我們](https://www.sigmaaldrich.com/TW/zh/life-science/about-us)[責任](https://www.sigmaaldrich.com/TW/zh/life-science/ssbi)[活動](https://www.sigmaaldrich.com/TW/zh/collections/events)[新聞稿](https://www.sigmaaldrich.com/TW/zh/collections/press)[節目](https://www.sigmaaldrich.com/TW/zh/life-science/partnership-programs)[工作機會](https://careers.merckgroup.com/global/en)[辦公室](https://www.sigmaaldrich.com/TW/zh/collections/offices) 社交媒體 [](https://www.linkedin.com/company/merck-life-science) [](https://x.com/Merck_lifesci) [](https://www.facebook.com/Merck.lifescience) [](https://www.instagram.com/mercklifescience/) [](https://www.youtube.com/@MerckLifeScience/featured) Merck __Research.開發。生產。__ 我們是全球生命科學產業的領導供應商，提供研究、生物技術開發與生產，以及藥物治療開發與生產的解決方案與服務。 [](https://www.sigmaaldrich.com/TW/zh) [Sigma-Aldrich® 溶液](https://www.sigmaaldrich.com/TW/zh/life-science/sigma-aldrich)[BioReliance® 解決方案](https://www.sigmaaldrich.com/TW/zh/life-science/bioreliance)[Millipore® 溶液](https://www.sigmaaldrich.com/TW/zh/life-science/millipore)[SAFC® 解決方案](https://www.sigmaaldrich.com/TW/zh/life-science/safc)[Milli-Q® 解決方案](https://www.sigmaaldrich.com/TW/zh/life-science/milliq)[Supelco® 解決方案](https://www.sigmaaldrich.com/TW/zh/life-science/supelco) © 2026 Merck KGaA, Darmstadt, Germany 和/或其附屬公司。版權所有，包括用於 AI 訓練的文字和資料挖掘以及類似技術。 未經許可，嚴禁複製本網站的任何資料。 [網站使用條款](https://www.sigmaaldrich.com/TW/zh/life-science/legal/site-use-terms)|[隱私權政策](https://www.sigmaaldrich.com/TW/zh/life-science/legal/privacy-statement)|[一般銷售條款](https://www.sigmaaldrich.com/TW/zh/life-science/legal/terms-and-conditions)|[版權同意書](https://www.sigmaaldrich.com/TW/zh/life-science/legal/copyright-consent)|[網站地圖](https://www.sigmaaldrich.com/TW/zh/site-map) An unknown error has occured.