MilliporeSigma

Immobilon® Tools

for Western blotting


Tailored for Your Blot

Western blotting success relies on having the right tools for the job. Our comprehensive Immobilon® portfolio offers what you need for dependable, reproducible, high sensitivity Western blotting.

Start with our selection of Immobilon® PVDF and nitrocellulose transfer membranes that allow you to choose the ideal membrane based on target protein size and detection method.

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  • Immobilon®-P membranes: Our classic PVDF membrane offers high protein binding for greater sensitivity as well as durability for stripping and re-probing. Compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-E membranes : The only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications and is compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-PSQ membranes: Higher binding capacity and retention for stronger signal, making these membranes ideal for protein sequencing and immunoblotting of low molecular weight proteins (<20 kDa).
  • Immobilon®-FL membranes: Developed for low background across a wide range of excitation and emission wavelengths for fluorescence-based detection protocols, these membranes are ideal for multiplexing, fluorescence, and chemifluorescence detection methods as well as non-fluorescent detection chemistries.
  • Immobilon® NC membranes: Our nitrocellulose membranes offer lowest background with decreased non-specific antibody binding and can bind both proteins and nucleic acids for a variety of blotting applications.
Immobilon NOW

The Best Thing Since Sliced Bread

Immobilon® NOW dispensers and rolls offer the convenience of pre-cut sheets with the flexibility of rolls in the formats you depend on.

  • Single-cut convenience
  • Compatibility with mini or midi gels
  • No waste
  • Compact design saves space in your lab
Immobilon® ECL Substrates for Superior Performance

Immobilon® ECL Substrates for Superior Performance

Highest sensitivity? Long signal duration? Immobilon® ECL (enhanced chemiluminescence) Western blotting HRP substrates were made to suit your needs.

  • Single component, ready-to-use formulations simplify detection for enhanced reproducibility and convenience
  • Two-component formulations for highest sensitivity and long signal, saving antibody in detection
  • A range of sensitivities to provide optimal signal-to-background ratio across a spectrum of target protein concentrations

30-Minute Immunodetection

Tired of immunodetection protocols that require hours of hands-on time? Accelerate immunodetection with the SNAP i.d.® 2.0 system for Western blotting. The SNAP i.d. 2.0 system uses a vacuum manifold to drive blocking reagents, antibodies, and wash buffers directly through the PVDF or nitrocellulose membrane, speeding immunodetection workflows.

  • Shortens time to results (from 4-24 hours to just 30 minutes)
  • Enables parallel processing of blots, making it easy to apply consistent conditions across experiments