MilliporeSigma

Immobilon® Tools

for Western blotting

Tailored for Your Blot

Western blotting success relies on having the right tools for the job. Our comprehensive Immobilon® portfolio offers what you need for dependable, reproducible, high sensitivity Western blotting.

Start with our selection of Immobilon® PVDF and nitrocellulose transfer membranes that allow you to choose the ideal membrane based on target protein size and detection method.

View our Offerings

  • Immobilon®-P membranes: Our classic PVDF membrane offers high protein binding for greater sensitivity as well as durability for stripping and re-probing. Compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-E membranes : The only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications and is compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-PSQ membranes: Higher binding capacity and retention for stronger signal, making these membranes ideal for protein sequencing and immunoblotting of low molecular weight proteins (<20 kDa).
  • Immobilon®-FL membranes: Developed for low background across a wide range of excitation and emission wavelengths for fluorescence-based detection protocols, these membranes are ideal for multiplexing, fluorescence, and chemifluorescence detection methods as well as non-fluorescent detection chemistries.
  • Immobilon® NC membranes: Our nitrocellulose membranes offer lowest background with decreased non-specific antibody binding and can bind both proteins and nucleic acids for a variety of blotting applications.
Immobilon NOW

The Best Thing Since Sliced Bread

Immobilon® NOW dispensers and rolls offer the convenience of pre-cut sheets with the flexibility of rolls in the formats you depend on.

  • Single-cut convenience
  • Compatibility with mini or midi gels
  • No waste
  • Compact design saves space in your lab
Immobilon® ECL Substrates for Superior Performance

Immobilon® ECL Substrates for Superior Performance

Highest sensitivity? Long signal duration? Immobilon® ECL (enhanced chemiluminescence) Western blotting HRP substrates were made to suit your needs.

  • Single component, ready-to-use formulations simplify detection for enhanced reproducibility and convenience
  • Two-component formulations for highest sensitivity and long signal, saving antibody in detection
  • A range of sensitivities to provide optimal signal-to-background ratio across a spectrum of target protein concentrations

30-Minute Immunodetection

Tired of immunodetection protocols that require hours of hands-on time? Accelerate immunodetection with the SNAP i.d.® 2.0 system for Western blotting. The SNAP i.d. 2.0 system uses a vacuum manifold to drive blocking reagents, antibodies, and wash buffers directly through the PVDF or nitrocellulose membrane, speeding immunodetection workflows.

  • Shortens time to results (from 4-24 hours to just 30 minutes)
  • Enables parallel processing of blots, making it easy to apply consistent conditions across experiments

Related Applications

Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.

Related Product Categories

Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Membrane Immunodetection Devices
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Electrophoresis Systems & Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.