Nanomaterial Bioconjugation Techniques

Nanomaterial Bioconjugation Techniques


The combination of nanomaterials and biomolecules is an exciting and rapidly developing area of biomedical and diagnostic innovation. This handbook serves as a guide for bioconjugation and surface modification of inorganic nanoparticles and provides detailed protocols for the conjugation of nanomaterials used in both current and future diagnostic applications. 

The handbook delves into the use of iron oxide nanoparticle conjugates in biomedical applications such as cell separation, drug delivery, magnetic hyperthermia, nanomagnetic gene transfection, and MRI. Additionally, it provides bioconjugation protocols for fluorescent nanoparticles with DNA and antibodies and examines the plasmonic properties of magnetic nanoparticle conjugates and their potential role in diagnostics, as well as conjugation protocols for newly developed nanostructures.

With a foreword by leading bioconjugation expert Dr. Greg Hermanson, the guide is organized for easy navigation into the three most commonly employed detection methods:

  • Magnetic imaging
  • Fluorescence imaging
  • Optical imaging

This handbook is a must-have for those working at the intersection of nanomaterials, engineering, and diagnostic development.

Featured Articles

"Biomedical Applications of Magnetic Micro- and Nanoparticles"
Author: Jon Dobson 

"Fluorescent Nanoparticles for Imagingand Diagnostics"
Authors: Maria Ada Malvindi, Enrico Binetti, Pier Paolo Pompa

"Plasmonic Materials and Their Applications in Diagnostics"
Authors: Bo Zhang, Jeyarama S. Ananta, Meijie Tang, Hongjie Dai 

"METHOD: Conjugating Gold Nanorods to Streptavidin and IgG"
Author: Christian Schoen 

"METHOD: Covalent Bioconjugation of Antibodies to Carboxyl Terminated Nanoparticles"
Authors: Rhea Decker, Steven J. Oldenburg

"METHOD: Conjugation Protocols for Magnetic Nanoparticles"
Authors: Xin Ji, Y. Andrew Wang

"METHOD: Fluorescent Silica Nanoparticle Bioconjugation"
Authors: Maria Ada Malvindi, Enrico Binetti, Pier Paolo Pompa

"METHOD: Conjugation of Proteins to Gold Nanoparticles"
Author: Benny Pacheco

"METHOD: Directional Antibody Conjugation of Silica-coated Gold Nanoparticles"
Authors: Justin Harris, Brantley Henson, Jason Cook, Kimberly Homan

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