HomeWebinarsMore Success in LC-MS – Tips and Tricks for Sample & Mobile Phase Preparation and Column Selection

More Success in LC-MS – Tips and Tricks for Sample & Mobile Phase Preparation and Column Selection


What Does It Cover?

LC-MS is one of the most commonly used analytical techniques in various sectors for quantitation and identification of unknown from variety of complex samples. Use of LC-MS has expanded over the years as it offers both selectivity and specificity in analysis. With advances in both chromatography and mass spectrometry, sensitivity and accuracy of this technique has further increased, allowing for detection and identification of low-level analytes in complex sample matrices.

The LC-MS workflow has three main components, which determine successful analyses: sample preparation, choice of mobile phase components and column selection. Not paying enough attention to one of these components can complicate data analysis, quantitation and identification.

In this seminar, you will learn critical factors to consider when selecting the sample preparation methods, mobile phase components and HPLC columns.

What Will You Learn?

We will discuss:

  • Different sample preparation methods and their effect on sample clean up (particle removal, analyte binding, and extractables)
    • Quality of mobile phase components (solvents, water and buffer salts) and their impact on background signal in LC-MS analysis
    • HPLC column selection
    • Useful tips to improve overall chromatography performance

Who Should Attend?

Scientists, technicians, PIs who use LC-MS and HPLC in drug development, pharmaceutical, proteomics, food analysis, environmental applications.


Vivek Joshi, Ph.D.

Vivek Joshi, Ph.D.


Applications and Market Adoption Manager

Vivek Joshi, Ph.D. is an Applications and Market Adoption Manager developing new products and applications for products that span analytical workflows, molecular and protein science workflows and cell culture workflows. Prior to joining us, Dr. Joshi worked on high throughput LC‐MS applications at ArQule. Dr. Joshi’s doctoral research focused on molecularly imprinted polymers (MIPs), and he completed postdoctoral studies of MIPs and capillary electrochromatography. Recently, Dr. Joshi was the presenter of a sample preparation module as part of the Drug Dissolution Training offered by the United States Pharmacopeia (USP).

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