Molecular Biology & Genomics Learning Hub

Quantitative PCR detection builds on basic PCR techniques and allows researchers to estimate the quantity of starting material in a sample with a much wider dynamic range.

Polymerase chain reaction is one of the most widely used techniques in molecular biology with 3 main steps: denature, anneal, and extend.

The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.

Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.

Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge.

CRISPR, miRNA, siRNA, and shRNA technologies for gene expression and gene silencing applications.

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

PCR troubleshooting guide for reliable data in molecular assays.

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions.