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Supelco

Chromolith® WP 300 Protein A 25-4.6 HPLC column

Pricing and availability is not currently available.

storage conditions

Store at +2°C to +8°C.

feature

endcapped: no

parameter

2 - 45 °C temp. range
200 bar pressure

application(s)

HPLC: suitable

matrix

highly porous
monolithic

pore size

2 μm macroporosity
30 nm mesoporosity

operating pH range

1.5 - 7.5

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificate of Analysis
Certificate of Origin
Articles
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.
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High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
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Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy
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Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy
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Chromolith® WP 300 Epoxy: Learn more about preparing the column, immobilization via epoxide functions and via Schiff base mechanism & use as HPLC column.
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