06105

Millipore

Bile esculin azide agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Enterococcus Selective Agar
NACRES:
NA.85
Pricing and availability is not currently available.

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
beef extract, 5.0 g/L
casein enzymic hydrolysate, 17.0 g/L
esculin, 1.0 g/L
ferric ammonium citrate, 0.5 g/L
oxgall, 10.0 g/L
proteose peptone, 3.0 g/L
sodium azide, 0.15 g/L
sodium chloride, 5.0 g/L

mfr. no.

NutriSelect® Plus

application(s)

microbe id | specific enzyme detection: suitable
microbiology: suitable (microbial identification)
water monitoring: suitable

Featured Industry

Clinical Testing
Environmental
Food and Beverages

suitability

selective and differential for Enterococcus spp.
selective and differential for Streptococcus spp.

Related Categories

General description

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

Application

Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.

Preparation Note

Suspend 56.65g in 1 l distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

Xn

Risk Statement

21/22-52/53

Safety Statement

36/37-61

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn.
Journal of Clinical Microbiology, 1(5) , 440-443 (1975)
Articles
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
Read More

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