69198

Millipore

Fraser Broth, Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Listeria Selective Enrichement Broth, Base (acc. Fraser)
NACRES:
NA.85
Pricing and availability is not currently available.

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 5.0 g/L
disodium phosphate dihydrate, 12.0 g/L
esculin, 1.0 g/L
lithium chloride, 3.0 g/L
meat extract, 5.0 g/L
peptic digest of animal tissue, 5.0 g/L
potassium dihydrogen phosphate, 1.35 g/L
sodium chloride, 20.0 g/L
yeast extract, 5.0 g/L

mfr. no.

NutriSelect® Plus

application(s)

microbiological culture: suitable
microbiology: suitable
pathogen testing: suitable
sample preparation: suitable

final pH

7.2±0.2 (25 °C)

Featured Industry

Agriculture
Environmental
Food and Beverages

suitability

selective and differential for Listeria spp.

Application

Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

Preparation Note

Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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