EMB Agar

suitable for microbiology, NutriSelect® Plus

Eosin Methylene Blue Agar

Quality Level





shelf life

limited shelf life, expiry date on the label


agar, 15.0 g/L
dipotassium hydrogen phosphate, 2.0 g/L
eosin Y, 0.4 g/L
lactose, 10.0 g/L
methylene blue, 0.065 g/L
peptone, 10.0 g/L


NutriSelect® Plus


microbiological culture: suitable
microbiology: suitable (microbial identification)

final pH

7.1±0.2 (25 °C)

Featured Industry

Cleaning Products
Clinical Testing
Food and Beverages
Personal Care


slightly selective and differential for Candida spp.
slightly selective and differential for Citrobacter spp.
slightly selective and differential for Enterobacter spp.
slightly selective and differential for Enterococcus spp.
slightly selective and differential for Escherichia coli
slightly selective and differential for Klebsiella spp.
slightly selective and differential for Proteus spp.
slightly selective and differential for Salmonella spp.
slightly selective and differential for Staphylococcus spp.
slightly selective and differential for coliforms
slightly selective and differential for enterobacteriaceae


A versatile solid medium for the differentiation of Eschericha coli and Aerobacter aerogenes, for identifcation of Candida albicans and for the identification of coagulase-positive Staphylococci.

Preparation Note

Suspend 37.5 g in 1 litre of distilled water. Bring to the boil to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidize the methylene blue and to suspend the precipitate.

Other Notes

Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
M. Levine
The Journal of Infectious Diseases, 23, 43-43 (1918)
Y Henis et al.
Microbial ecology, 17(2), 171-180 (1989-03-01)
The population of a strain ofEscherichia coli that was resistant to nalidixic acid and streptomycin declined rapidly in samples of sterile and nonsterile Cayuga Lake water and reached an undetectable level in nonsterile water at 24 and 72 hours when...
Shuaiguo Zhao et al.
Lab on a chip, 19(6), 941-947 (2019-02-01)
Microfluidic-based portable devices for stool analysis are important for detecting established biomarkers for gastrointestinal disorders and understanding the relationship between gut microbiota imbalances and various health conditions, ranging from digestive disorders to neurodegenerative diseases. However, the challenge of processing stool...
J.E. Holt-Harris et al.
The Journal of Infectious Diseases, 18, 596-596 (1916)
Isolation and identification of coagulase-positive Staphylococci on Levine's eosin-methylene blue agar.
The Journal of laboratory and clinical medicine, 56, 908-910 (1960-12-01)
Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.
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