88597

Millipore

Hydrogen peroxide solution

3%, suitable for microbiology

Synonym(s):
Catalase Test
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein/REAXYS Number:
3587191
MDL number:
PubChem Substance ID:
NACRES:
NA.85
Pricing and availability is not currently available.

Quality Level

Agency/Method

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

vapor pressure

23.3 mmHg ( 30 °C)

assay

2.8-3.2% (redox titration)

shelf life

limited shelf life, expiry date on the label

concentration

1-5%

application(s)

microbe id | specific enzyme detection: suitable
microbiology: suitable (microbial identification)

mp

0.0 °C

density

1.000 g/cm3

Featured Industry

Clinical Testing
Environmental
Food and Beverages
Veterinary

suitability

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Application

Hydrogen peroxide solution has been used:
  • to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
  • for the induction of apoptosis in cultured human microvascular endothelial cells
  • as a substrate for superoxide dismutase

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
Choi S and Love PE
Bio-protocol, 8(1) (2018)
Superoxide is the critical driver of DOPAL autoxidation, lysyl adduct formation, and crosslinking of alpha-synuclein
Werner-Allen JW, et al.
Biochemical and Biophysical Research Communications, 487(2), 281-286 (2017)
Bach1 induces endothelial cell apoptosis and cell-cycle arrest through ROS generation
Wang X, et al.
Oxidative Medicine and Cellular Longevity, 2016(1) (2016)
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice...
James R Van Brocklyn et al.
Cancer research, 74(19), 5364-5370 (2014-08-12)
Glioblastoma remains a devastating disease for which novel therapies are urgently needed. Here, we report that the Aurora-A kinase inhibitor alisertib exhibits potent efficacy against glioblastoma neurosphere tumor stem-like cells in vitro and in vivo. Many glioblastoma neurosphere cells treated...
Articles
On the Trail of Campylobacter - The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis. However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness. Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar fl agella
Read More
Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.
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For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.
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Sigma-Aldrich.com presents an article concerning Differentiation of <i>Escherichia coli</i> from coliforms.
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Protocols
In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.
Read More

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