C6152

Sigma-Aldrich

Carmine

certified by the Biological Stain Commission

Synonym(s):
Cochineal, Alum lake of carminic acid, Nacarat, Natural Red 4
CAS Number:
Colour Index Number:
75470
EC Number:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content: minimum has not been established

application(s)

hematology: suitable
histology: suitable

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)

InChI key

DGQLVPJVXFOQEV-UHFFFAOYSA-N

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General description

Carmine is a red compound and is commonly used with aluminum, iron or other metal salts to enhance its activity. During smear preparation, carmine is required for chromatin staining in fresh material. In presence of aluminum chloride, it is required for the staining of glycogen.

Application

Carmine has been used for the staining of mammary glands. It has also been used for the staining of chromosomes.

Packaging

25 g in glass bottle

Suitability

Certified for use in Orth′s lithium carbonate technique, Southgate′s mucicarmine method, and Best′s carmine for glycogen

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Genetic background affects susceptibility to mammary hyperplasias and carcinomas in Apc(min)/+ mice.
Moser AR, et al.
Cancer Research, 61, 3480-3480 (2001)
Precocious differentiation of the virgin Wistar-Kyoto rat mammary gland.
Benton ME, et al.
Endocrinology, 140, 2659-2659 (1999)
Florence Charlot et al.
Nucleic acids research, 42(19), 11965-11978 (2014-09-28)
The eukaryotic RecA homologue Rad51 is a key factor in homologous recombination and recombinational repair. Rad51-like proteins have been identified in yeast (Rad55, Rad57 and Dmc1), plants and vertebrates (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3 and DMC1). RAD51 and DMC1 are...
Methods in Mammary Gland Biology and Breast Cancer Research (2000)
Sonia de Assis et al.
Journal of visualized experiments : JoVE, (44), DOI: 10-DOI: 10 (2010-10-26)
Studies in rodent models of breast cancer show that exposures to dietary/hormonal factors during the in utero and pubertal periods, when the mammary gland undergoes extensive modeling and re-modeling, alter susceptibility to carcinogen-induced mammary tumors. Similar findings have been described...

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