01494

Sigma-Aldrich

Nancy-520

greener alternative

suitable for RT-PCR, ≥97.0% (HPCE)

EC Number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

assay

≥97.0% (HPCE)

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

solubility

DMSO: soluble

fluorescence

λex 520 nm; λem 554 nm in TE buffer; DNA

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA.
Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

WGK Germany

WGK 2

Flash Point(F)

188.6 °F - closed cup - Solvent

Flash Point(C)

87 °C - closed cup - Solvent

Certificate of Analysis
Certificate of Origin
  1. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  2. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  3. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  4. What is the mutagenic potential of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?

    The Ames test (II) has confirmed that Product 01494, Nancy-520, is less mutagenic than SYBR green I and ethidium bromide.

  5. What is the sensitivity of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?

    Product 01494, Nancy-520, shows a higher sensitivity than ethidium bromide and a slightly higher sensitivity to SYBR Green I.

  6. Does Product 01494, Nancy-520, require the same safety handling that is required for Ethidium bromide?

    No, the handling of Product 01494, Nancy-520, does not require the strict safety instructions that are needed for Ethidium bromide. Normal latex gloved may be used. No specific "Nitrile" gloves are required. No particularly equipped fume hood for Nancy-520 is required. Other work can be done in the same fume hood, where Nancy-520 is used.

  7. Can Product 01494, Nancy-520 staining solution, be reused?

    Re-use of Product 01494, Nancy-520 staining solution, is not recommended.

  8. Can Nancy-520 be detected when using the Odyssey® Fc imager?

    According to an application note published by LI-COR "Imaging Nucleic Acid Gels on the Odyssey® Fc Imager," published in 2011, Nancy-520 was successfully tested in the Fc imager, using the 600 channel.®Odyssey is a registered Trademark of LI-COR, Inc. The trademark belongs to their respective owners. The Odyssey® Fc Imaging system is covered by U.S. patents, foreign equivalents, and patents pending.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nolan, T. and Bustin, S.A.
PCR Technology: Current Innovations (2013)
Filion, M.
Quantitative Real-time PCR in Applied Microbiology (2012)
Park, D.J.
PCR Protocols (2011)
Rodriguez-Lazaro, D.
Real-Time PCR in Food Science: Current Technology and Applications (2013)
Wilks, M.
PCR Detection of Microbial Pathogens (2012)
Articles
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
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Nancy-520 for DNA Detection and Quantitation
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