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λex 520 nm; λem 554 nm in TE buffer; DNA
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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The Ames test (II) has confirmed that Product 01494, Nancy-520, is less mutagenic than SYBR green I and ethidium bromide.
Product 01494, Nancy-520, shows a higher sensitivity than ethidium bromide and a slightly higher sensitivity to SYBR Green I.
No, the handling of Product 01494, Nancy-520, does not require the strict safety instructions that are needed for Ethidium bromide. Normal latex gloved may be used. No specific "Nitrile" gloves are required. No particularly equipped fume hood for Nancy-520 is required. Other work can be done in the same fume hood, where Nancy-520 is used.
Re-use of Product 01494, Nancy-520 staining solution, is not recommended.
According to an application note published by LI-COR "Imaging Nucleic Acid Gels on the Odyssey® Fc Imager," published in 2011, Nancy-520 was successfully tested in the Fc imager, using the 600 channel.®Odyssey is a registered Trademark of LI-COR, Inc. The trademark belongs to their respective owners. The Odyssey® Fc Imaging system is covered by U.S. patents, foreign equivalents, and patents pending.
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