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Actinomycin D

for fluorescence, ≥90% (HPLC), from Streptomyces sp.

Dactinomycin, Actinomycin IV, Actinomycin C1
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

biological source

Streptomyces sp.


for fluorescence


≥90% (HPLC)




red, powder




H2O: 0.5 mg/mL
DMSO: 1 mg/mL
acetone: 10 mg/mL
acetonitrile: 10 mg/mL


λex 420; λem 443 in ethanol

Mode of action

DNA synthesis | interferes

antibiotic activity spectrum


storage temp.


SMILES string




InChI key


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General description

Chemical structure: peptide


Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.


Product is packaged in a bottomless glass bottle with the contents inside an inserted fused cone.


This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Other Notes

Conditions for safe storage, including any incompatibilities. Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.

Certificate of Analysis

Certificate of Origin

Thomas Schwarzl et al.
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Y Maeda et al.
Journal of molecular biology, 215(2), 321-329 (1990-09-20)
The effect of intercalating drugs (the anthracycline group of antibiotics, ethidium bromide, actinomycin D) on stepwise melting of DNA was studied by differential scanning calorimetry (DSC). The DSC DNA melting profile of plasmid pJL3-TB5 DNA (5277 base-pairs in length) consists...
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