Atto 495

BioReagent, suitable for fluorescence, ≥98.0% (HPCE)

MDL number:
PubChem Substance ID:

Quality Level

product line



≥98.0% (HPCE)


DMF: soluble
DMF: soluble
DMSO: soluble
H2O: soluble
H2O: soluble
acetone: soluble
acetone: soluble


λex 499 nm; λem 526 nm in 0.1 M phosphate pH 7.0


suitable for fluorescence

storage temp.


SMILES string




InChI key


General description

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.


Atto 495 is a lipophilic fluorescent compound, which has served as the neutral analyte in the model, along with SDS as the charged micelle acting as a carrier to transport this solute in an electric field. Atto 495 was included as fluorescence EOF marker for chip electrophoresis. This reagent is zwitterionic at pH 8.3 and thus migrates as a neutral substance under the proper conditions.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Victor U Weiss et al.
Analytical chemistry, 82(19), 8146-8152 (2010-09-02)
Permeabilization of model lipid membranes by virus-derived peptides, viral proteins, and entire virions of human rhinovirus was assessed by quantifying the release of a fluorescent dye from liposomes via a novel chip electrophoretic assay. Liposomal leakage readily occurred upon incubation...
Gerhard Bilek et al.
Electrophoresis, 30(24), 4292-4299 (2009-12-17)
In first attempts at elucidating the transfer of the RNA genome of a human Rhinovirus through lipid membranes in vitro we made use of liposomes decorated with recombinant receptors. This model system was characterized previously by CE but suffered from...
Adolfo Téllez et al.
Electrophoresis, 30(2), 357-364 (2009-01-06)
The effect of formamide (FA) as a modifier on the retention in MEKC with SDS as the detergent was investigated. The mobility of a series of alkylphenones and of a zwitterionic fluorescent compound as a function of the FA and...
Adolfo Téllez et al.
Electrophoresis, 29(18), 3916-3923 (2008-10-14)
Three equilibria determine the interaction of a neutral analyte with the detergent in micellar electrokinetic chromatography and therefore its migration: (i) that of the free analyte in the aqueous phase with the micelle, (ii) its association with free detergent monomers...
H. Maas, G. Calzaferri
The Spectrum, 16, 18-18 (2003)

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