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17866

Sigma-Aldrich

Streptavidin−CryptoFluor Crimson

suitable for fluorescence

Pricing and availability is not currently available.

mol wt

Mr ~40200 (αα′β2)(lit.)

fluorescence

λex 585 nm; λem ~658 nm in 0.1 M phosphate pH 7.2

suitability

suitable for fluorescence

storage temp.

2-8°C

General description

The CryptoFluor dyes are phycobiliproteins isolated from cryptomonad algae that provide additional spectral characteristics to complement the more common phycobiliproteins (e.g., R-PE and APC) as well as providing much smaller molecular weights (around 40,000 Daltons). These characteristics are meant to provide additional functionality to the phycobiliprotein pigments when it comes to multiplexing assays or to getting dyes into the cell.

Application

Streptavidin-CryptoFluor Crimson is used to label biotin-conjugated (biotinylated) molecules and biotin derivatized surfaces in a wide variety of immunofluorescence applications such as immunochemistry, histochemistry and flow cytometry. Streptavidin-CryptoFluor Crimson may be used in the development of bioassay devices such as Lab-on-a-chip (LoC) systems and microarrays.

Packaging

One vial contents 0.5 mg of conjugate and ~11 mg additives (see reconstitution)

Reconstitution

Reconstitute to 250 μl with destilled or deionized water (the solution will consist of 100 mM sodium phosphate pH 7.2, 50 mM sucrose, 0.05% sodium azide, 150 mM Sodium chloride, 0.1 % BSA and 2 mg/ml pigment). Do not freeze, store at 4°C in the dark.

Legal Information

CryptoFluor is a trademark of Martek Biosciences Corp.
Certificate of Analysis
Certificate of Origin
W G Telford et al.
Cytometry, 44(1), 16-23 (2001-04-20)
Phycobiliproteins play an important role in fluorescent labeling, particularly for flow cytometry. The spectral properties of R-phycoerythrin (R-PE) and allophycocyanin (APC) have made them the dominant reagents in this class of fluorochromes. In this study, we evaluate a lesser-known but...

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