21878

Sigma-Aldrich

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym(s):
FLUOS, 5(6)-FAM SE
Empirical Formula (Hill Notation):
C25H15NO9
CAS Number:
Molecular Weight:
473.39
MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥80% (HPLC)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in DMF

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

InChI key

YVTPVJDECOUTJW-UHFFFAOYSA-N

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Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.
Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.
Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Seyedeh Masoumeh Ghoreishi et al.
International journal of nanomedicine, 13, 4671-4683 (2018-08-30)
Nowadays, molecular imaging radiopharmaceuticals', nanoparticles', and/or small-molecule biomarkers' applications are increasing rapidly worldwide. Thus, researchers focus on providing the novel, safe, and cost-effective ones. In the present experiment, technetium-99m (99mTc)-labeled PEG-citrate dendrimer-G2 conjugated with glutamine (nanoconjugate) was designed and assessed...
Federica De Santis et al.
PloS one, 14(3), e0213150-e0213150 (2019-03-05)
In nature, many plants or their extracted compounds have been found to possess anti-inflammatory features and therapeutic properties against infectious as well as non-infectious diseases, including cancer. In this study, we analysed the immunomodulatory effects on innate immune cells of...
null
G P Vigers et al.
The Journal of cell biology, 107(3), 1011-1024 (1988-09-01)
We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously...
M Lu-Steffes et al.
Clinical chemistry, 28(11), 2278-2282 (1982-11-01)
Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 microL of...
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