5(6)-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Empirical Formula (Hill Notation):
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DMF: soluble
acetonitrile: soluble


λex 575 nm; λem 605 nm in 0.1 M phosphate pH 7.0


suitable for fluorescence

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5(6)-Carboxy-X-rhodamine N-succinimidyl ester may used as a labeling reagent to attach the fluorophore 5(6)-carboxy-X-rhodamine to target molecules such as nucleic acids. 5(6)-Carboxy-X-rhodamine labeled molecules may be used in fluorescence energy transfer (FRET) applications.

Certificate of Analysis

Certificate of Origin

S C Hung et al.
Analytical biochemistry, 252(1), 78-88 (1997-11-05)
We have synthesized and characterized the spectroscopic properties of 56 energy transfer (ET) fluorescent dye-labeled primers differing in (i) the spacing between the donor and acceptor, (ii) the nature of the spacer (either oligonucleotide or polydideoxyribose phosphate), (iii) the primer...
S C Hung et al.
Analytical biochemistry, 255(1), 32-38 (1998-02-04)
Fluorescence energy transfer (ET) primers are far superior to single dye-labeled primers as labels for DNA sequencing and polymerase chain reaction amplification. We compare here ET primers with different donor and acceptor dye combinations with respect to the relative acceptor...
Tae Seok Seo et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(17), 5926-5931 (2005-04-15)
We report four-color DNA sequencing by synthesis (SBS) on a chip, using four photocleavable fluorescent nucleotide analogues (dGTP-PC-Bodipy-FL-510, dUTP-PC-R6G, dATP-PC-ROX, and dCTP-PC-Bodipy-650) (PC, photocleavable; Bodipy, 4,4-difluoro-4-bora-3alpha,4alpha-diaza-s-indacene; ROX, 6-carboxy-X-rhodamine; R6G, 6-carboxyrhodamine-6G). Each nucleotide analogue consists of a different fluorophore attached to...
Hailong Li et al.
Biosensors & bioelectronics, 27(1), 167-171 (2011-07-26)
In this article, we report on the first use of fluorescence resonance energy transfer (FRET) dye-labeled probe for fluorescence resonance enhanced DNA detection to greatly improve discrimination ability toward single-base mismatch using conjugation polymer poly(p-phenylenediamine) nanobelts (PNs) as a sensing...

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