25455

Sigma-Aldrich

4-Chloro-7-nitrobenzofurazan

suitable for fluorescence, BioReagent, ≥97.0% (HPLC)

Synonym(s):
NBD-chloride, 4-Chloro-7-nitro-1,2,3-benzoxadiazole
Empirical Formula (Hill Notation):
C6H2ClN3O3
CAS Number:
Molecular Weight:
199.55
Beilstein/REAXYS Number:
614212
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥97.0% (HPLC)

mp

97-100 °C
97-99 °C (lit.)

fluorescence

λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

suitability

suitable for fluorescence

SMILES string

O-N+(=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

IGHBXJSNZCFXNK-UHFFFAOYSA-N

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Application

4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

Xi

Risk Statement

36/38

Safety Statement

26

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis
Certificate of Origin
P D Bragg et al.
Biochimica et biophysica acta, 1413(3), 159-171 (1999-11-11)
Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is...
Parallel dip-pen nanolithography using spore- and colloid-terminated cantilevers.
Marcus A Kramer et al.
Small (Weinheim an der Bergstrasse, Germany), 8(24), 3791-3794 (2012-08-18)
Junjie Yao et al.
NeuroImage, 64, 257-266 (2012-09-04)
We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which...
Rob van Zwieten et al.
Biochimica et biophysica acta, 1821(12), 1493-1500 (2012-09-11)
Maintenance of the asymmetric distribution of phospholipids across the plasma membrane is a prerequisite for the survival of erythrocytes. Various stimuli have been shown to induce scrambling of phospholipids and thereby exposure of phosphatidylserine (PS). In two types of patients...
Emmanuel C Gorospe et al.
Gastrointestinal endoscopy, 76(5), 933-938 (2012-09-18)
There are currently 2 existing confocal laser endomicroscopy (CLE) platforms: probe-based CLE (pCLE) and endoscope-based CLE (eCLE) systems, each with its own criteria for identifying dysplasia in Barrett's esophagus (BE). The diagnostic performance of these 2 systems has not been...

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