37291

Sigma-Aldrich

Dihydroethidium

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym(s):
Hydroethidine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
Beilstein/REAXYS Number:
5107482
MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥95% (HPCE)

solubility

acetonitrile: soluble
methanol: soluble

fluorescence

λex 358 nm; λem 461 nm
λex 392 nm; λem 410 nm in methanol

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Linkage

B-ring reduced analog of ethidium

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Karina A Roth et al.
Journal of endodontics, 38(9), 1253-1256 (2012-08-16)
Root canal fillings are intended to prevent microbial proliferation over time in the canal after treatment. The objective of this study was to assess biofilm proliferation within the sealer-dentin interfaces of 2 methacrylate resin-based systems, self-etch (SE) and total-etch (TE)...
Francisco R M Laurindo et al.
Methods in enzymology, 441, 237-260 (2008-06-17)
Assessment of low-level superoxide in nonphagocytic cells is crucial for assessing redox-dependent signaling pathways and the role of enzymes such as the NADPH oxidase complex. However, most superoxide probes present inherent limitations. Particularly, assessment of dihydroethidium (DHE) fluorescence is limited...
Anna Rita Fetoni et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(9), 4011-4023 (2013-03-01)
This study addresses the relationship between cochlear oxidative damage and auditory cortical injury in a rat model of repeated noise exposure. To test the effect of increased antioxidant defenses, a water-soluble coenzyme Q10 analog (Qter) was used. We analyzed auditory...
Rossarin Suwanarusk et al.
The Journal of antimicrobial chemotherapy, 70(1), 124-129 (2014-08-26)
Methylene blue, once discarded due to its unsettling yet mild side effects, has now found a renewed place in the pharmacopoeia of modern medicine. The continued spread of drug-resistant Plasmodium vivax and Plasmodium falciparum has also led to a recent...
Hai-Yan Jin et al.
PloS one, 7(6), e38502-e38502 (2012-06-14)
Inflammation and oxidative stress play a crucial role in angiotensin (Ang) II-mediated vascular injury. Angiotensin-converting enzyme 2 (ACE2) has recently been identified as a specific Ang II-degrading enzyme but its role in vascular biology remains elusive. We hypothesized that loss...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.