5-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level

product line



DMF: soluble
acetonitrile: soluble


λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0


suitable for fluorescence

storage temp.


SMILES string




InChI key



5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Other Notes

Labeling reagent for prepn. of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing


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Hazard Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Xia Xiang et al.
Analytica chimica acta, 751, 155-160 (2012-10-23)
The development of simple and inexpensive DNA detection strategy is very significant for droplet-based microfluidic system. Here, a droplet-based biosensor for multiplexed DNA analysis is developed with a common imaging device by using fluorescence-based colorimetric method and a graphene nanoprobe....
Ting Qiu et al.
The Analyst, 135(9), 2394-2399 (2010-08-03)
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively charged dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a strategy...
Patrick J Finn et al.
Nucleic acids research, 30(13), 2877-2885 (2002-06-28)
A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage over existing reagents in that no purification is required to remove unreacted nucleotide or associated breakdown products prior...
Yunfeng Xia et al.
The Analyst, 137(24), 5705-5709 (2012-10-27)
We report a sensitive approach for SERS detection of cytochrome c using target binding-induced conformational changes of signal transduction probe (STP). STP labeled with a SERS-active molecule, carboxy-X-rhodamine (ROX), is immobilized on the substrate where the formation of a rigid...
Jonathan F Lovell et al.
Journal of the American Chemical Society, 133(46), 18580-18582 (2011-10-27)
Photodynamic therapy (PDT) stands to benefit from improved approaches to real-time treatment monitoring. One method is to use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it (via caspase detection). Here, we report porphyrins as...
Fluorescent Labeling of Peptides
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