42424

Sigma-Aldrich

Atto 550

for fluorescence, ≥90% (HPCE)

MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

grade

for fluorescence

assay

≥90% (HPCE)

fluorescence

λex 554 nm; λem 574 nm in 0.1 M phosphate pH 7.0

storage temp.

−20°C

General description

Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3® and is characterized by a high photostability.

find more information
here

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
CAB-O-SIL is a registered trademark of Cabot Corp.
Cy3 is a trademark of Cytiva

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Sebastian Braun et al.
PloS one, 6(5), e19125-e19125 (2011-06-01)
Nucleic acids promote amyloid formation in diseases including Alzheimer's and Creutzfeldt-Jakob disease. However, it remains unclear whether the close interactions between amyloid and nucleic acid allow nucleic acid secondary structure to play a role in modulating amyloid structure and function....
Eric J White et al.
Clinical chemistry, 55(12), 2121-2129 (2009-10-10)
Epidemiologic studies require identification or typing of microbial strains. Macrorestriction DNA mapping analyzed by pulsed-field gel electrophoresis (PFGE) is considered the current gold standard of genomic typing. This technique, however, is difficult to implement because it is labor-intensive and difficult...
Michel Frigoli et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(33), 8319-8330 (2009-07-04)
Core-shell type dual fluorescent nanoparticles (NPs) in the 16 nm diameter range with a selective ligand (cyclam) attached to the surface and two fluorophores--9,10-diphenyl-anthracene (donor, D) and pyrromethene PM 567 (acceptor, A)--embedded within the polymer core were synthesized and their...

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