43429

Sigma-Aldrich

Atto 635 NHS ester

BioReagent, suitable for fluorescence, ≥80% (coupling to amines)

Empirical Formula (Hill Notation):
C32H38ClN3O8
Molecular Weight:
628.11
MDL number:
PubChem Substance ID:

product line

BioReagent

assay

≥80% (coupling to amines)

fluorescence

λex 624 nm; λem 655 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[O-]Cl(=O)(=O)=O.CC1=CC(C)(C)N(CCCC(=O)ON2C(=O)CCC2=O)c3cc4c(C=C5C=C\C(C=C5C4(C)C)=[N+](/C)C)cc13

InChI

1S/C32H38N3O4.ClHO4/c1-20-19-31(2,3)34(14-8-9-30(38)39-35-28(36)12-13-29(35)37)27-18-26-22(16-24(20)27)15-21-10-11-23(33(6)7)17-25(21)32(26,4)5;2-1(3,4)5/h10-11,15-19H,8-9,12-14H2,1-7H3;(H,2,3,4,5)/q+1;/p-1

InChI key

TZOJKHWYZZHQDJ-UHFFFAOYSA-M

Application

Atto 635 NHS is a new label for proteins and oligo-nucleotides with high molecular absorption (120.000) and quantum yield (0.25) as well as sufficient stoke′s shift (excitation maximum 635 nm, emission maximum 659 nm). This label is optimized for use with diode laser excitation at 635 nm and characterized by high photostability. Atto 635 NHS ester is quantitatively activated and offers an exceptional high coupling efficiency.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Christopher A Teske et al.
The journal of physical chemistry. B, 109(28), 13811-13817 (2006-07-21)
Confocal laser scanning microscopy (CLSM) is being increasingly used for observing protein uptake in porous chromatography resins. Recent CLSM studies have revealed the possible existence of a nondiffusive protein transport mechanism. Observing protein uptake with CLSM requires labeling the protein...
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were...

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