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DPX Mountant for histology is regarded as a permanent or a resinous mounting media.
No, this mounting media is not soluble in water.
Xylene is the solvent of choice for dissolving DPX Mountant.
DPX dries rapidly in comparison to either Canada balsam or gum dammar.
Dehydration refers to removing the water by using a series of grades of alcohols. Typically slides spend 1-5 minutes each in a series of alcohols. Sequences vary by laboratory. A typical sequence would be 1 station of 80% alcohol, 2-3 changes of 95% alcohol, 2-3 changes of absolute alcohol. The slides are then processed through 2-3 changes of xylene. After finishing with the last xylene solution, the slides are ready for coverslipping with the DPX. In some special stain procedures, the time in the dehydration alcohols is shortened to reduce the possibility of the dye leaching from the stained slides during dehydration. Typically 10-15 rapid dips in each alcohol is suitable. The purpose of dehydration is to remove water from the tissue sections. If the coverslipped slides are clear, the water was removed. If the coverslipped slides appear cloudy, some water was retained.
The product specifications allow for a refractive index between 1.518 to 1.520.
Tissue typically has an average refractive index of 1.53 or 1.54. Most permanent mounting medias will have a refractive index ranging from 1.51 to 1.55. Water by comparison has a refractive index of only 1.33. The closer the refractive index of the tissue and the mounting media, the greater the clarity and resolution of the slide when viewed under a microscope.
Yes. It is possible to remove the coverslip once the DPX has set. Typically the slide is immersed in Xylene. The coverslip may loosen in several minutes, several hours or after 24 hours or greater. Differences may occur due to the amount of mounting media originally applied at the time of coverslipping or the length of time the DPX was allowed to harden. At times it might be necessary to gently pry the edges of the coverslip. This may shorten the period needed for the coverslip to detach. The slide is then agitated in 3 or 4 changes of xylene. The slides are then processed through 2-3 changes of absolute alcohol, 2-3 changes of 95% alcohol. The slides are then rinsed well in running tap water. The slides are now suitable for destaining and restaining.
Yes. Both products are considered permanent mounting medias. They are used for similar purposes.
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