Eosin 5-isothiocyanate

≥95.0% (UV)

Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level


≥95.0% (UV)


λex 521; λem 544 (pH 9.0)

storage temp.


SMILES string




InChI key


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Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Other Notes

Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies


Health hazard

Signal Word


Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
T Chiba et al.
Biochimica et biophysica acta, 897(1), 14-24 (1987-02-12)
The characteristics of the anion transport system in human erythrocyte, which can be modified by eosin 5-isothiocyanate (EITC), were studied using the pH titration method and by measuring the sulfate efflux. Based on the pH dependence of EITC binding to...
S Q Liu et al.
The American journal of physiology, 264(5 Pt 1), C1155-C1164 (1993-05-01)
Although eosin-5-maleimide (EM) covalently labels band 3 and has been thought to react at the external-facing anion transport site, EM reversibly inhibits Cl- exchange at 0 degrees C in a noncompetitive fashion, indicating that under these conditions it does not...
Valeria Levi et al.
Biochimica et biophysica acta, 1599(1-2), 141-148 (2002-12-14)
Self-association of bovine serum albumin (BSA) was explored using fluorescence resonance energy transfer (FRET) between two populations of the protein labeled separately with either fluorescein-5'-isothiocyanate (FITC) or eosin-5'-isothiocyanate (EITC). The energy transfer reached the steady state after 5 s at...
Ryan R Hansen et al.
Analytical and bioanalytical chemistry, 392(1-2), 167-175 (2008-07-29)
Quantitative evaluation of minimal polynucleotide concentrations has become a critical analysis among a myriad of applications found in molecular diagnostic technology. Development of high-throughput, nonenzymatic assays that are sensitive, quantitative and yet feasible for point-of-care testing are thus beneficial for...
S Papp et al.
Biophysical journal, 51(2), 205-220 (1987-02-01)
Ca2+-ATPase molecules were labeled in intact sarcoplasmic reticulum (SR) vesicles, sequentially with a donor fluorophore, fluorescein-5'-isothiocyanate (FITC), and with an acceptor fluorophore, eosin-5'-isothiocyanate (EITC), each at a mole ratio of 0.25-0.5 mol/mol of ATPase. The resonance energy transfer was determined...
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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