Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

EtDi, EthD-1
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level


~90% (HPCE)


≥250 °C (lit.)


DMF: soluble
DMSO: soluble
H2O: soluble


λex 493 nm in H2O
λex 528 nm; λem 617 nm in TE buffer; DNA


suitable for fluorescence

storage temp.


SMILES string




InChI key


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Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.


Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes


Risk Statement


Safety Statement



UN 3096 4.3(8) / PGII

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Takeichiro Nakane et al.
Scientific reports, 7, 45641-45641 (2017-04-04)
The current study describes a scalable, porous large-format engineered cardiac tissue (LF-ECT) composed of human induced pluripotent stem cells (hiPSCs) derived multiple lineage cardiac cells with varied 3D geometries and cell densities developed towards the goal of scale-up for large...
Nai-Chen Cheng et al.
Acta biomaterialia, 51, 258-267 (2017-01-31)
Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is...
Susanne Radonjic-Hoesli et al.
The Journal of allergy and clinical immunology, 140(6), 1632-1642 (2017-04-17)
Eosinophils are a subset of granulocytes that can be involved in the pathogenesis of different diseases, including allergy. Their effector functions are closely linked to their cytotoxic granule proteins. Release takes place through several different mechanisms, one of which is...
Yong Mao et al.
Acta biomaterialia, 94, 330-339 (2019-06-10)
Human amniotic membrane (AM) has intrinsic anti-inflammatory, anti-fibrotic and antimicrobial properties. Tissue preservation methods have helped to overcome the short shelf life of fresh AM allowing "on demand" use of AM grafts. Cryopreserved AM that retains all native tissue components...
Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids.
J Markovits et al.
Analytical biochemistry, 94(2), 259-264 (1979-04-15)
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